基于生物条形码法的凝血酶电化学适体传感器
Amplified electrochemical aptasensor for thrombin based on bio-barcode method.
作者信息
Zhang Xiaoru, Qi Baoping, Li Ying, Zhang Shusheng
机构信息
Key Laboratory of Eco-chemical Engineering, Ministry of Education, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao 266042, China.
出版信息
Biosens Bioelectron. 2009 Sep 15;25(1):259-62. doi: 10.1016/j.bios.2009.06.026. Epub 2009 Jun 24.
In the present study, an electrochemical aptasensor for highly sensitive detection of thrombin was developed based on bio-barcode amplification assay. For this proposed aptasensor, capture DNA aptamerI was immobilized on the Au electrode. The functional Au nanoparticles (DNA-AuNPs) are loaded with barcode binding DNA and aptamerII. Through the specific recognition for thrombin, a sandwich format of Au/aptamerI/thrombin/DNA-AuNPs was fabricated. After hybridization with the PbSNPs-labeled barcode DNA, the assembled sensor was obtained. The concentration of thrombin was monitored based on the concentration of lead ions dissolved through differential pulse anodic stripping voltammetric (DPASV). Under optimum conditions, a detection limit of 6.2x10(-15) mol L(-1) (M) thrombin was achieved. In addition, the sensor exhibited excellent selectivity against other proteins.
在本研究中，基于生物条形码扩增分析开发了一种用于高灵敏度检测凝血酶的电化学适体传感器。对于这种提出的适体传感器，捕获DNA适体I固定在金电极上。功能性金纳米颗粒（DNA-AuNPs）负载有条形码结合DNA和适体II。通过对凝血酶的特异性识别，构建了Au/适体I/凝血酶/DNA-AuNPs的夹心结构。与PbSNPs标记的条形码DNA杂交后，得到组装好的传感器。基于通过差分脉冲阳极溶出伏安法（DPASV）溶解的铅离子浓度监测凝血酶浓度。在最佳条件下，凝血酶的检测限达到6.2×10⁻¹⁵ mol L⁻¹（M）。此外，该传感器对其他蛋白质表现出优异的选择性。
Zotero 插件