McKeague Maureen, Derosa Maria C
Department of Chemistry, Carleton University, 1125 Colonel By Drive, Ottawa, ON, Canada K1S 5B6.
J Nucleic Acids. 2012;2012:748913. doi: 10.1155/2012/748913. Epub 2012 Oct 24.
Aptamers are single-stranded oligonucleotides that bind to targets with high affinity and selectivity. Their use as molecular recognition elements has emerged as a viable approach for biosensing, diagnostics, and therapeutics. Despite this potential, relatively few aptamers exist that bind to small molecules. Small molecules are important targets for investigation due to their diverse biological functions as well as their clinical and commercial uses. Novel, effective molecular recognition probes for these compounds are therefore of great interest. This paper will highlight the technical challenges of aptamer development for small molecule targets, as well as the opportunities that exist for their application in biosensing and chemical biology.
适配体是与靶标具有高亲和力和选择性的单链寡核苷酸。它们作为分子识别元件的应用已成为生物传感、诊断和治疗的一种可行方法。尽管有这种潜力，但与小分子结合的适配体相对较少。小分子因其多样的生物学功能以及临床和商业用途，是重要的研究靶标。因此，针对这些化合物的新型有效分子识别探针备受关注。本文将重点介绍针对小分子靶标开发适配体的技术挑战，以及它们在生物传感和化学生物学中的应用机会。
