Terrer Elodie, Raskin Anne, Koubi Stephen, Dionne Alexandro, Weisrock Gauthier, Sarraquigne Caroline, Mazuir Alain, Tassery Hervé
Department of Restorative Dentistry, Marseille Dental School, University of the Mediterranean.
J Contemp Dent Pract. 2010 Jan 1;11(1):E095-102.
A new and innovative therapeutic concept using a light-induced fluorescence evaluator for diagnosis and treatment (LIFEDT) of dental caries based on the imaging and autofluorescence of dental tissues is proposed. The aims of this series of in vivo experiments are to compare and analyze the brightness variations of sound dentin and active and arrested carious dentin illuminated with an intraoral LED camera and to determine if this new device could be helpful in daily practice to discriminate between caries and sound dentin.
A new intraoral LED camera that emits visible blue light was used in this in vivo study to illuminate and photograph 15 teeth at high magnification. The magnified images were examined using the free Image J V version 1.41 software. Four standardized rectangular areas were drawn on each picture that included both healthy and pathologic areas to analyze variations in brightness using a brightness formula: L = 0.299 Red + 0.587 Green + 0.114 Blue.
Statistically significant differences in the brightness were found between active and arrested caries processes in an area of infected dentin designated Z2. Within the limitations of this in vivo study, the images created with the intraoral LED camera revealed significant variations in fluorescence between sound dentin and active and arrested caries processes.
The LIFEDT concept provides a therapeutic concept based on these findings of variations in fluorescence between healthy and pathologic tissue.
This concept defines a pragmatic clinical and therapeutic approach for treating active and arrested carious lesions based on the interpretation of variations of a fluorescence signal and applying the LIFEDT concept to the treatment of dentin carious lesions.
提出一种基于牙体组织成像和自发荧光的龋病诊断与治疗用光诱导荧光评估仪(LIFEDT)的全新创新治疗理念。这一系列体内实验的目的是比较和分析用口腔内发光二极管(LED)相机照射时,健康牙本质、活动龋和静止龋牙本质的亮度变化,并确定这种新设备在日常实践中是否有助于区分龋病和健康牙本质。
在这项体内研究中,使用一种能发出可见蓝光的新型口腔内LED相机,以高倍放大对15颗牙齿进行照射和拍照。使用免费的Image J V 1.41版软件检查放大后的图像。在每张图片上绘制四个标准化的矩形区域,这些区域包括健康和病变区域,使用亮度公式L = 0.299红色 + 0.587绿色 + 0.114蓝色来分析亮度变化。
在指定为Z2的感染牙本质区域,活动龋和静止龋过程之间的亮度存在统计学上的显著差异。在这项体内研究的局限性内,用口腔内LED相机生成的图像显示,健康牙本质与活动龋和静止龋过程之间的荧光存在显著差异。
LIFEDT理念基于健康组织和病变组织之间荧光变化的这些发现提供了一种治疗理念。
该理念定义了一种实用的临床和治疗方法,用于基于荧光信号变化的解读来治疗活动龋和静止龋病变,并将LIFEDT理念应用于牙本质龋病变的治疗。