EA4203 Laboratory of Biology and Nano-science, Université Montpellier 1, Montpellier, France.
Clin Oral Investig. 2013 Apr;17(3):757-63. doi: 10.1007/s00784-012-0770-9. Epub 2012 Aug 2.
Our aim was to determine the origin of the red fluorescence of carious dentine observed with the Soprolife® camera.
We conducted in vitro studies to evaluate the origin of the red fluorescence using acids and matrix metalloproteinase (MMP) to mimic caries and methylglycoxal (MGO) to evaluate the effect of glycation reactions on the red fluorescence. In every step of these models, we detected the changes of dentin photonic response with Soprolife® in daylight mode and in treatment mode. A Raman spectroscopy analysis was performed to determine the variations of the dentin organic during the in vitro caries processes. Raman microscopy was performed to identify change in the collagen matrix of dentine.
The red fluorescence observed in carious dentine using a Soprolife® camera corresponds to the brownish color observed using daylight. Demineralization using nitric acid induces a loss of the green fluorescence of dentine. The red fluorescence of carious dentine is resistant to acid treatment. Immersion of demineralized dentine in MGO induces a change of color from white to orange-red. This indicates that the Maillard reaction contributes to lesion coloration. Immersion of demineralized dentine in an MMP-1 solution followed by MGO treatment results in a similar red fluorescence. Raman microspectroscopy analysis reveals accumulation of AGE's product in red-colored dentine.
Our results provide important information on the origin of the fluorescence variation of dentine observed with the Soprolife® camera. We demonstrate that the red fluorescence of carious dentine is linked to the accumulation of Advanced Glycation End products (AGE).
The study provides a new biological basis for the red fluorescence of carious dentine and reinforces the importance of the Soprolife® camera in caries diagnostics.
本研究旨在确定 Soprolife® 相机观察到的龋损牙本质红色荧光的来源。
我们进行了体外研究，使用酸和基质金属蛋白酶（MMP）来模拟龋损，并使用甲基乙二醛（MGO）来评估糖化反应对红色荧光的影响，以评估红色荧光的来源。在这些模型的每一步中，我们都使用 Soprolife® 在日光模式和治疗模式下检测牙本质光子反应的变化。进行拉曼光谱分析以确定牙本质有机成分在体外龋损过程中的变化。进行共聚焦激光扫描显微镜下的拉曼显微镜检查以鉴定牙本质胶原基质的变化。
Soprolife® 相机观察到的龋损牙本质中的红色荧光与日光下观察到的棕褐色相对应。使用硝酸进行脱矿作用会导致牙本质绿色荧光的丧失。龋损牙本质的红色荧光对酸处理具有抗性。将脱矿牙本质浸泡在 MGO 中会导致颜色从白色变为橙红色。这表明美拉德反应有助于病变着色。将脱矿牙本质浸泡在 MMP-1 溶液中，然后再用 MGO 处理，会导致类似的红色荧光。拉曼微光谱分析显示，红色牙本质中积累了 AGE 的产物。
我们的研究结果为 Soprolife® 相机观察到的牙本质荧光变化的来源提供了重要信息。我们证明了龋损牙本质的红色荧光与晚期糖基化终产物（AGE）的积累有关。
本研究为龋损牙本质的红色荧光提供了新的生物学基础，并强调了 Soprolife® 相机在龋病诊断中的重要性。
