Duong Tan Nhut, Nguyen Thanh Hai, Mai Xuan Phan
Plant Molecular Biology and Plant Breeding Laboratory, Tay Nguyen Institute of Biology, Dalat, Lam Dong, Vietnam.
Methods Mol Biol. 2010;589:15-20. doi: 10.1007/978-1-60327-114-1_2.
A protocol for micropropagation of begonia was established utilizing a thin cell layer (TCL) system. This system has been employed to produce several thousand shoots per sample. Explant size and position, and plant growth regulators (PGRs) contribute to the tissue morphogenesis. By optimizing the size of the tissue and applying an improved selection procedure, shoots were elongated in 8 weeks of culture, with an average number of 210 +/- 9.7 shoots per segment. This system has facilitated a number of studies using TCL as a model for micropropagation and will enable the large-scale production of begonia. On an average, the best treatment would allow production of about 10,000 plantlets by the micropropagation of the axillary buds of one plant with five petioles, within a period of 8 months.
利用薄细胞层（TCL）系统建立了秋海棠微繁殖方案。该系统已被用于每个样本生产数千个芽。外植体的大小和位置以及植物生长调节剂（PGR）有助于组织形态发生。通过优化组织大小并应用改进的选择程序，芽在培养8周后伸长，每段平均有210±9.7个芽。该系统促进了许多以TCL为微繁殖模型的研究，并将实现秋海棠的大规模生产。平均而言，最佳处理方法可在8个月内通过一株有五片叶柄的植物腋芽微繁殖生产约10000株幼苗。
