Bradaï Fatiha, Sánchez-Romero Carolina
Departamento de Botánica y Fisiología Vegetal, Universidad de Málaga, Campus de Teatinos s/n, 29071 Málaga, Spain.
Plants (Basel). 2021 Feb 19;10(2):396. doi: 10.3390/plants10020396.
Cryopreservation is considered the best technique for the safe, long-term conservation of embryogenic cultures. However, before integrating it into a somatic embryogenesis system, the influence of cryopreservation on the final production of plants should be investigated. The objective of this investigation was to evaluate the effect of cryopreservation on the regeneration performance of olive embryogenic cultures as well as on the quality of the plants obtained and their response to ex vitro establishment. In order to analyze the influence of the genotype, all the investigations were carried out in two genetically distinct embryogenic lines. The results obtained revealed no variation in the regeneration potential or the quality of the regenerated plants due to cryopreservation. The subsequent multiplication, rooting, and acclimatization steps were not influenced by cryopreservation either, although a significant genotype × cryopreservation interaction was found for shoot length during the multiplication step. The genotype played an important role, determining the quality of the regenerated plants and some aspects of the multiplication and rooting phases. This investigation revealed that the droplet-vitrification procedure optimized for the cryopreservation of olive somatic embryos can be efficiently used for the long-term conservation of olive embryogenic lines.
冷冻保存被认为是安全、长期保存胚性培养物的最佳技术。然而，在将其整合到体细胞胚胎发生系统之前，应研究冷冻保存对植物最终产量的影响。本研究的目的是评估冷冻保存对橄榄胚性培养物再生性能的影响，以及对所获得植物的质量及其离体驯化反应的影响。为了分析基因型的影响，所有研究均在两个遗传上不同的胚性系中进行。所得结果表明，冷冻保存对再生潜力或再生植物的质量没有影响。随后的增殖、生根和驯化步骤也不受冷冻保存的影响，尽管在增殖步骤中发现了基因型×冷冻保存对茎长的显著相互作用。基因型起着重要作用，决定了再生植物的质量以及增殖和生根阶段的一些方面。本研究表明，针对橄榄体细胞胚冷冻保存优化的玻璃化滴冻法可有效地用于橄榄胚性系的长期保存。
