澳大利亚本土观赏植物扇叶草的离体繁殖

In vitro propagation of Australian native ornamental plant, Scaevola.

作者信息

Wong Chui Eng, Bhalla Prem L

机构信息

Plant Molecular Biology and Biotechnology laboratory, Australian Research Centre of Excellence for Integrative Legume Research, Faculty of Land and Food Resources, The University of Melbourne, Parkville, VIC, Australia.

出版信息

Methods Mol Biol. 2010;589:235-41. doi: 10.1007/978-1-60327-114-1_22.

DOI:10.1007/978-1-60327-114-1_22

PMID:20099106

Abstract

In this chapter, we describe a robust method for the micropropagation of Australian fan flower, Scaevola, a native plant increasingly being used in the ornamental horticulture industry. Shoot segments from different species of Scaevola can be successfully multiplied following this protocol. Multiple shoots can be obtained in hormone-free Murashige and Skoog medium. The regenerated shoot is rooted on hormone-free medium within 4-6 weeks. In vitro grown plantlets readily adapt to glasshouse conditions.

摘要

在本章中，我们描述了一种用于澳大利亚扇花（Scaevola）微繁殖的可靠方法，Scaevola是一种本土植物，越来越多地用于观赏园艺行业。按照此方案，来自不同Scaevola物种的茎段能够成功增殖。在不含激素的Murashige和Skoog培养基中可以获得多个芽。再生芽在4至6周内在不含激素的培养基上生根。在体外培养的幼苗很容易适应温室条件。