Pati Pratap Kumar, Kaur Navtej, Sharma Madhu, Ahuja Paramvir Singh
Department of Biotechnology, Guru Nanak Dev University, Amritsar, India.
Methods Mol Biol. 2010;589:163-76. doi: 10.1007/978-1-60327-114-1_16.
In vitro propagation of rose is an important tool for rapid multiplication and development of new cultivars with desirable traits. However, successful in vitro propagation requires an understanding of specific requirements and precise manipulation of various factors. Efficient protocols for different stages of micropropagation using apical buds or nodal segments are currently available. Recently, new challenges for refinements of protocols for high rate of shoot multiplication and development of cost effective methods has gained importance. Significance of the liquid static culture for shoot proliferation and root induction for rose has also gained prominence. Other distinct approaches of in vitro propagation include organogenesis and embryogenesis. These approaches are important for the successful implementation of various biotechnological techniques used for rose improvement programmes. Types of explants, media and optimization of conditions are major factors for successful regeneration of rose.
玫瑰的离体繁殖是快速繁殖和培育具有理想性状新品种的重要手段。然而，成功的离体繁殖需要了解特定要求并精确控制各种因素。目前已有使用顶芽或节段进行微繁殖不同阶段的高效方案。最近，完善用于高芽增殖率的方案以及开发经济高效方法的新挑战变得愈发重要。液体静置培养对玫瑰芽增殖和生根诱导的重要性也日益凸显。离体繁殖的其他不同方法包括器官发生和胚胎发生。这些方法对于成功实施用于玫瑰改良计划的各种生物技术至关重要。外植体类型、培养基和条件优化是玫瑰成功再生的主要因素。
