Winkelmann Traud
Institute of Floriculture and Woody Plant Science, Tree Nursery Science Section, Leibniz University Hannover, Hannover, Germany.
Methods Mol Biol. 2010;589:281-90. doi: 10.1007/978-1-60327-114-1_26.
Cyclamen (Cyclamen persicum) is an economically important ornamental pot plant with local use as cut flower as well. Traditionally, it is propagated via seeds, but interest is given in vegetative propagation of parental lines as well as superior single plants. Somatic embryogenesis is an efficient in vitro propagation method for many cyclamen cultivars. Starting from ovules of unpollinated flowers, callus is induced and propagated in a medium containing 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-(gamma,gamma-dimethylallylamino)purine (2iP). Transfer to hormone-free medium results in the differentiation of somatic embryos, which afterwards germinate on the same medium. These first culture stages take about 6-7 months and are carried out in complete darkness. Two to four months after the transfer to light, plantlets develop which can be acclimatized in the greenhouse. The regenerated plants are characterized by low percentages of somaclonal variation. This protocol has proven useful not only for clonal propagation, but also for artificial seed preparation, cryopreservation, genetic transformation and protoplast regeneration.
仙客来（仙客来）是一种具有重要经济价值的观赏盆栽植物，也可作为切花用于当地市场。传统上，仙客来通过种子繁殖，但目前人们对亲本系以及优良单株的营养繁殖也很感兴趣。体细胞胚胎发生是许多仙客来品种高效的离体繁殖方法。从未授粉花的胚珠开始，在含有2,4-二氯苯氧乙酸（2,4-D）和6-（γ，γ-二甲基烯丙基氨基）嘌呤（2iP）的培养基中诱导并增殖愈伤组织。转移到无激素培养基中会导致体细胞胚胎分化，随后这些胚胎在相同的培养基上萌发。这些最初的培养阶段大约需要6-7个月，并且在完全黑暗的条件下进行。转移到光照下2至4个月后，形成可在温室中驯化的幼苗。再生植株的体细胞无性系变异百分率较低。该方案不仅已被证明对克隆繁殖有用，而且对人工种子制备、冷冻保存、遗传转化和原生质体再生也有用。
