弗氏完全佐剂和不完全佐剂、制备方法以及实验动物使用的质量控制标准。
Freund's complete and incomplete adjuvants, preparation, and quality control standards for experimental laboratory animals use.
作者信息
Stewart-Tull Duncan E S
机构信息
Division of Infection and Immunity, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow, Scotland.
出版信息
Methods Mol Biol. 2010;626:59-72. doi: 10.1007/978-1-60761-585-9_5.
Quality control and quality assurance procedures are discussed for the agreed benchmark standard Freund's complete adjuvant (FCA). In addition, the use of the incomplete adjuvant (FIA) in the preparation of antisera is discussed. A major problem is the use of a safe and suitable mineral oil in FCA and FIA; manufacturers should provide infra-red spectra and gas liquid chromatography analyses. A range of safety tests, toxicity, pyrogenicity and endotoxin assays and advice on practical procedures for the use of these adjuvants are described.
针对已商定的基准标准弗氏完全佐剂（FCA），讨论了质量控制和质量保证程序。此外，还讨论了不完全佐剂（FIA）在抗血清制备中的使用。一个主要问题是在FCA和FIA中使用安全且合适的矿物油；制造商应提供红外光谱和气液色谱分析。描述了一系列安全测试、毒性、热原性和内毒素测定，以及关于这些佐剂使用实际程序的建议。
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