Commissariat à l'Energie Atomique Saclay, IRAMIS, SIS2M/LSDRM, Gif-sur-Yvette, France.
Magn Reson Med. 2010 Feb;63(2):269-74. doi: 10.1002/mrm.22231.
High-resolution magic-angle spinning has become a valuable tool to study biologic samples; however, there are only a few high-resolution magic-angle spinning methodologies that have been explored specifically to deal with small semisolid biomaterials such as tissues. Here, we report a novel high-resolution (1)H NMR spectroscopic approach using magic-angle coil spinning. We demonstrate its potential for intact tissues by combining a resonant microcoil with a large-volume commercial magic-angle spinning rotor, where the microcoil offers incredible sensitivity and the large-volume magic-angle spinning rotor provides stable slow magic-angle spinning. This approach provides high-resolution spectra with high sensitivity and the preservation of living biologic specimens for an accurate chemical analysis.
高分辨率魔角旋转已成为研究生物样本的有力工具；然而，仅有少数几种高分辨率魔角旋转方法专门用于处理小的半固态生物材料，如组织。在这里，我们报告了一种新颖的使用魔角线圈旋转的高分辨率 (1)H NMR 光谱方法。我们通过将共振微线圈与大容量商用魔角旋转转子相结合，证明了它在完整组织中的应用潜力，其中微线圈提供了难以置信的灵敏度，大容量魔角旋转转子提供了稳定的慢速魔角旋转。这种方法提供了具有高灵敏度和生物样本保存的高分辨率光谱，可用于准确的化学分析。
