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代谢组学研究中的核磁共振技术：应用实例快速概述

NMR Techniques in Metabolomic Studies: A Quick Overview on Examples of Utilization.

作者信息

Kruk Joanna, Doskocz Marek, Jodłowska Elżbieta, Zacharzewska Anna, Łakomiec Joanna, Czaja Kornelia, Kujawski Jacek

机构信息

Department of Organic Chemistry, Faculty of Pharmacy, Poznan University of Medical Sciences, Grunwaldzka 6 Str., 60-780 Poznan, Poland.

RootInnovation Sp. z o.o., Jana Matejki 11 Str., 50-333 Wrocław, Poland.

出版信息

Appl Magn Reson. 2017;48(1):1-21. doi: 10.1007/s00723-016-0846-9. Epub 2016 Nov 2.

DOI:10.1007/s00723-016-0846-9
PMID:28111499
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5222922/
Abstract

Metabolomics is a rapidly developing branch of science that concentrates on identifying biologically active molecules with potential biomarker properties. To define the best biomarkers for diseases, metabolomics uses both models (in vitro, animals) and human, as well as, various techniques such as mass spectroscopy, gas chromatography, liquid chromatography, infrared and UV-VIS spectroscopy and nuclear magnetic resonance. The last one takes advantage of the magnetic properties of certain nuclei, such as H, C, P, F, especially their ability to absorb and emit energy, what is crucial for analyzing samples. Among many spectroscopic NMR techniques not only one-dimensional (1D) techniques are known, but for many years two-dimensional (2D, for example, COSY, DOSY, JRES, HETCORE, HMQS), three-dimensional (3D, DART-MS, HRMAS, HSQC, HMBC) and solid-state NMR have been used. In this paper, authors taking apart fundamental division of nuclear magnetic resonance techniques intend to shown their wide application in metabolomic studies, especially in identifying biomarkers.

摘要

代谢组学是一门快速发展的科学分支，专注于识别具有潜在生物标志物特性的生物活性分子。为了确定疾病的最佳生物标志物，代谢组学使用模型（体外、动物）和人类，以及各种技术，如质谱、气相色谱、液相色谱、红外和紫外可见光谱以及核磁共振。最后一种技术利用某些原子核的磁性，如氢、碳、磷、氟，特别是它们吸收和发射能量的能力，这对于分析样品至关重要。在许多核磁共振光谱技术中，不仅有一维（1D）技术为人所知，而且多年来二维（2D，例如，COSY、DOSY、JRES、HETCORE、HMQS）、三维（3D，DART-MS、HRMAS、HSQC、HMBC）和固态核磁共振技术也已被使用。在本文中，作者剖析了核磁共振技术的基本分类，旨在展示其在代谢组学研究中的广泛应用，尤其是在识别生物标志物方面。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d25a/5222922/b22609afc910/723_2016_846_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d25a/5222922/8e608c218fcb/723_2016_846_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d25a/5222922/b7dedbd37ed4/723_2016_846_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d25a/5222922/04a43dc27b73/723_2016_846_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d25a/5222922/b22609afc910/723_2016_846_Fig11_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d25a/5222922/8e608c218fcb/723_2016_846_Fig8_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d25a/5222922/b7dedbd37ed4/723_2016_846_Fig9_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d25a/5222922/04a43dc27b73/723_2016_846_Fig10_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d25a/5222922/b22609afc910/723_2016_846_Fig11_HTML.jpg

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3
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