染料木黄酮对卵巢癌细胞相关微小RNA的调控作用
Modulation of microRNA associated with ovarian cancer cells by genistein.
作者信息
Parker L P, Taylor D D, Kesterson J, Metzinger D S, Gercel-Taylor C
机构信息
Division of Gynecologic Oncology, Department of Obstetrics, Gynecology and Women's Health University of Louisville School of Medicine, Louisville, KY 40202, USA.
出版信息
Eur J Gynaecol Oncol. 2009;30(6):616-21.
PURPOSE
Role of microRNAs in malignancies is well established due their regulatory role in cellular differentiation, proliferation and cell cycle control. Our purpose was to determine miRNA profiles of serially established ovarian cancer cell lines and the effect of genistein treatment.
METHODS
Cell lines (UL-3A, UL-3B) were established from one patient during progression of disease. miRNA profiling was performed in untreated and genistein-treated cells. Estrogen receptors (ER) were studied with real-time polymerase chain reaction (RT-PCR) and Western immunoblotting. In vitro migration and invasion assays were utilized.
RESULTS
While 108 miRNAs were expressed equally in both cell lines and their genistein-treated counterparts, an additional 53 miRNAs were differentially expressed. Genistein resulted in induction of ERalpha and ERbeta in ovarian cancer cells. A significant reduction in migration and invasion of UL-3A and UL-3B was demonstrated in genistein-treated cells.
CONCLUSION
Common and unique miRNA profiles were demonstrated between the two cell lines, some of which were altered by genistein.
目的
由于微小RNA在细胞分化、增殖和细胞周期控制中发挥调节作用，其在恶性肿瘤中的作用已得到充分证实。我们的目的是确定连续建立的卵巢癌细胞系的微小RNA谱以及染料木黄酮处理的效果。
方法
在一名患者疾病进展过程中建立细胞系（UL-3A、UL-3B）。对未处理和经染料木黄酮处理的细胞进行微小RNA谱分析。通过实时聚合酶链反应（RT-PCR）和蛋白质免疫印迹法研究雌激素受体（ER）。采用体外迁移和侵袭试验。
结果
虽然108种微小RNA在两个细胞系及其经染料木黄酮处理的对应细胞系中表达相同，但另外53种微小RNA存在差异表达。染料木黄酮导致卵巢癌细胞中ERα和ERβ的诱导。在经染料木黄酮处理的细胞中，UL-3A和UL-3B的迁移和侵袭显著减少。
结论
在两个细胞系之间证实了常见和独特的微小RNA谱，其中一些因染料木黄酮而改变。
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