Department of Bioengineering, University of California, San Diego, La Jolla, California 92093-0412, USA.
Tissue Eng Part A. 2010 Jun;16(6):2017-27. doi: 10.1089/ten.TEA.2009.0768.
Following ischemic injury in the heart, little to no repair occurs, causing a progressive degeneration of cardiac function that leads to congestive heart failure. Cardiac tissue engineering strategies have focused on designing a variety of injectable scaffolds that range in composition from single-component materials to complex extracellular matrix (ECM)-derived materials. In this study, the pericardial ECM, a commonly used biomaterial, was investigated for use as an injectable scaffold for cardiac repair. It was determined that a solubilized form of decellularized porcine pericardium could be injected and induced to gel in vivo, prompting investigation with human pericardium, which has the decided advantage of offering an autologous therapy. Characterization showed that the matrix gels retained components of the native pericardial ECM, with extant protein and glycosaminoglycan content identified. The results of an in vitro migration assay indicate that the porcine pericardial matrix is a stronger chemoattractant for relevant cell types, but in vivo results showed that the two materials caused statistically similar amounts of neovascularization, demonstrating feasibility as injectable treatments. Potential stem cell mobilization was supported by the presence of c-Kit+ cells within the matrix injection regions. With this work, the pericardium is identified as a novel source for an autologous scaffold for treating myocardial infarction.
在心脏发生缺血性损伤后，几乎没有修复发生，导致心脏功能逐渐退化，进而引发充血性心力衰竭。心脏组织工程策略的重点是设计各种可注射支架，其组成范围从单一成分材料到复杂的细胞外基质 (ECM) 衍生材料。在这项研究中，研究了心包 ECM，一种常用的生物材料，将其用作心脏修复的可注射支架。结果表明，脱细胞猪心包的可溶解形式可以注射并在体内诱导凝胶化，这促使人们研究具有明显优势的人心包，因为人心包可提供自体治疗。特性分析表明，基质凝胶保留了天然心包 ECM 的成分，鉴定出存在蛋白质和糖胺聚糖。体外迁移实验的结果表明，猪心包基质对相关细胞类型具有更强的趋化作用，但体内结果表明，两种材料引起的新生血管化程度相似，这表明它们作为可注射治疗方法具有可行性。基质注射区域内存在 c-Kit+细胞，这支持了潜在的干细胞动员作用。通过这项工作，心包被确定为治疗心肌梗死的新型自体支架的来源。
