来自可育和不明原因不育男性的新鲜射出精子及经Percoll梯度离心沉淀精子中的胆固醇和磷脂水平。
Levels of cholesterol and phospholipids in freshly ejaculated sperm and Percoll-gradient-pelletted sperm from fertile and unexplained infertile men.
作者信息
Sugkraroek P, Kates M, Leader A, Tanphaichitr N
机构信息
Department of Obstetrics and Gynecology, Loeb Institute for Medical Research, Ottawa Civic Hospital, Ontario, Canada.
出版信息
Fertil Steril. 1991 Apr;55(4):820-7.
Cholesterol and phospholipid levels were determined in individual sperm samples obtained from 20 fertile and 20 unexplained infertile men. The determination was performed on both washed freshly ejaculated sperm and Percoll-gradient-pelletted sperm. Although sperm cholesterol levels in unexplained infertile patients were significantly lower, i.e., 10.6 +/- 1.3 (mean +/- SD) nmol/10(7) freshly ejaculated sperm and 5.4 +/- 0.7 nmol/10(7) Percoll-gradient-pelletted sperm as compared with 19.9 +/- 1.9 nmol/10(7) and 12.6 +/- 1.5 nmol/10(7) for corresponding sperm populations in fertile donors. Motility parameters measured in 10 sperm samples of the two groups of fertile and unexplained infertile men revealed increases in the amplitude of lateral head displacement and decreases in percent of straightness in sperm tracks from unexplained infertile men.
对从20名生育能力正常的男性和20名不明原因不育男性获取的个体精子样本进行了胆固醇和磷脂水平测定。测定在新鲜射出的洗涤精子和经Percoll梯度离心沉淀的精子上均进行。尽管不明原因不育患者的精子胆固醇水平显著较低，即新鲜射出精子为10.6±1.3（均值±标准差）nmol/10⁷，经Percoll梯度离心沉淀的精子为5.4±0.7 nmol/10⁷，而生育能力正常供者相应精子群体分别为19.9±1.9 nmol/10⁷和12.6±1.5 nmol/10⁷。对两组生育能力正常和不明原因不育男性的10个精子样本进行的活力参数测量显示，不明原因不育男性精子头部侧向位移幅度增加，精子轨迹直线度百分比降低。
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