采用上游法从新鲜和冻融后的射精精液中回收活动精子。

Motile sperm recovery from fresh and frozen-thawed ejaculates using a swim-up procedure.

作者信息

Graczykowski J W, Siegel M S

机构信息

Department of Obstetrics and Gynecology, University of Southern California-Los Angeles.

出版信息

Fertil Steril. 1991 Apr;55(4):841-3. doi: 10.1016/s0015-0282(16)54261-7.

DOI:10.1016/s0015-0282(16)54261-7

PMID:2010014

Abstract

The recovery of motile sperm in swim-ups from fresh semen that was washed and centrifuged one time was 33% and after 2 washes and centrifugations was 22%. The recovery rates for frozen-thawed semen were only 7% and 5%, respectively. The straight line velocity of sperm was increased after sperm swim-up, whereas changes to the lateral sperm head movement were not as evident. However, the dramatic loss in motile sperm numbers especially after swim-up from thawed semen indicate that more efficient methods of isolating motile sperm from thawed semen may be required.

摘要

从经一次洗涤和离心的新鲜精液中通过上游法回收的活动精子比例为33%，经两次洗涤和离心后为22%。冻融精液的回收率分别仅为7%和5%。精子上游后直线运动速度增加，而精子头部横向运动的变化不那么明显。然而，活动精子数量的显著减少，尤其是解冻精液上游后的减少，表明可能需要更有效的从解冻精液中分离活动精子的方法。