Department of General Surgery, Institute of Medical Sciences, Banaras Hindu University, Varanasi - 221 005, India.
Can J Surg. 2010 Feb;53(1):6-10.
Chronic nonhealing wounds are difficult to manage. Various substances are being used to heal these wounds. We sought to observe the effects of autologous epidermal cell suspension dressings on chronic nonhealing ulcers.
We enrolled patients of the wound clinic at University Hospital, Varanasi, India, with nonhealing wounds of more than 6 weeks' duration. We treated the wound beds with sterile dressings and antibiotics until the swab cultures became sterile. We prepared autologous epidermal cell suspensions from skin grafts and used them on the ulcer beds along with Vaseline gauze dressings. Follow-up visits with patients occurred weekly for assessment of wound healing and other changes.
Fifteen patients enrolled in our study. Of these, 6 patients had completely healed at 12 weeks, 1 patient at 16 weeks and 2 patients at 20 weeks after treatment. We observed a slow healing response in 6 patients, of whom 1 patient had healed completely at 32 weeks and another at 48 weeks. One patient needed skin grafting, and 3 patients were lost to follow-up.
Autologous noncultured epidermal cell suspension transplantation seems to be an effective, simple and time-saving method to treat chronic nonhealing wounds.
慢性难愈性创面难以处理。各种物质被用于治疗这些伤口。我们旨在观察自体表皮细胞悬浮液敷料对慢性难愈性溃疡的疗效。
我们招募了印度瓦拉纳西大学医院创面门诊的患者，这些患者的创面超过 6 周未愈。我们用无菌敷料和抗生素处理创面床，直到拭子培养无菌。我们从皮肤移植中制备自体表皮细胞悬浮液，并将其与凡士林纱布敷料一起用于溃疡床。每周对患者进行随访，评估创面愈合和其他变化。
我们的研究共纳入 15 名患者。其中，6 名患者在治疗 12 周时完全愈合，1 名患者在 16 周时愈合，2 名患者在 20 周时愈合。我们观察到 6 名患者的愈合反应较慢，其中 1 名患者在 32 周时完全愈合，另 1 名患者在 48 周时愈合。1 名患者需要植皮，3 名患者失访。
自体非培养表皮细胞悬浮液移植似乎是一种有效、简单且省时的治疗慢性难愈性创面的方法。
