Laboratory of Reproductive Biology and The Fertility Clinic, Department of Clinical Genetics, Copenhagen University Hospital, Rigshospitalet, Copenhagen, Denmark.
Fertil Steril. 2010 Nov;94(6):2186-90. doi: 10.1016/j.fertnstert.2009.11.032. Epub 2010 Jan 25.
To systematically search for leukemic cells in cryopreserved ovarian cortex from Danish female patients with leukemia, who had ovarian cortex cryopreserved for fertility preservation before potentially sterilizing treatment.
Retrospective analysis of data in a clinical project.
University hospital laboratories.
PATIENT(S): In total, 26 patients diagnosed with leukemia, who had ovarian tissue cryopreserved before potentially sterilizing chemotherapy and conditioning.
INTERVENTION(S): Ovarian cortex from each patient was examined with histology and immunohistochemistry. In addition, in eight cases a specific chromosomal abnormality could be used as a genetic marker for detection of malignant cells by polymerase chain reaction (PCR).
MAIN OUTCOME MEASURE(S): Evidence of malignant cells by immunohistochemistry or PCR.
RESULT(S): Histology and immunohistochemistry did not reveal malignant cell infiltration in the ovarian cortex of any of the patients. In six of the eight patients (75%) with chromosomal abnormalities in the malignant cells, PCR showed evidence of leukemic cells in the ovarian tissue.
CONCLUSION(S): Immunohistochemistry was unable to locate leukemic cells in the ovarian cortex; however, PCR detected potentially malignant cells in the majority of cases. The viability and malignancy of these cells remains to be determined. At present, reimplantation of ovarian cortex to leukemia patients cannot be recommended.
系统性搜索来自丹麦女性白血病患者的冷冻卵巢皮质中的白血病细胞，这些患者在接受潜在的绝育治疗前进行了卵巢皮质冷冻以保存生育能力。
临床项目数据的回顾性分析。
大学医院实验室。
共有 26 名被诊断患有白血病的患者，她们在进行潜在的化疗和预处理前进行了卵巢组织冷冻。
对每位患者的卵巢皮质进行组织学和免疫组织化学检查。此外，在 8 例中，可以使用特定的染色体异常作为聚合酶链反应（PCR）检测恶性细胞的遗传标记。
免疫组织化学或 PCR 检测到恶性细胞的证据。
组织学和免疫组织化学未显示任何患者的卵巢皮质中有恶性细胞浸润。在 8 例具有恶性细胞染色体异常的患者中，有 6 例（75%）的 PCR 显示卵巢组织中有白血病细胞的证据。
免疫组织化学无法定位卵巢皮质中的白血病细胞；然而，PCR 在大多数情况下检测到潜在的恶性细胞。这些细胞的活力和恶性程度仍有待确定。目前，不能向白血病患者推荐重新植入卵巢皮质。
