Institute for Craniofacial and Reconstructive Surgery, Providence Hospital, Southfield, MI, USA.
J Plast Reconstr Aesthet Surg. 2010 Sep;63(9):1553-6. doi: 10.1016/j.bjps.2009.08.011. Epub 2010 Jan 25.
The Mcfarlane flap or dorsal pedicled flap has become the standard model for pedicled rat skin flap study but its reliability has been called to question. In the past, there were possible confounding variable with the McFarlane flap and various methods were used to adjust these variables. We have developed a new model for studying skin flap necrosis and its prevention that eliminates these confounding variables.
The flap is a significant modification of the McFarlane flap where we form a blind ended pedicled tube using a 3 cm x 9 cm dorsal flap. Survival area is measured using digital photography and computer assisted analysis. This new flap is compared with the standard McFarlane flap with n=25 in each group.
The mean survival area of the new flap (15.673 cm(2)+SD3.37) is comparable with the McFarlane flap (18.904 cm(2)+SD3.79). The relative merit lies in the elimination of the confounding variable of the graft bed influence on our flap without a significant reduction in the survival area.
A new rat model is presented that may be used in studying the effect of various treatment modalities on pedicled skin flaps. This model has the benefit of eliminating graft bed effect without the risk of flap and wound infection or desiccation that have been encountered using other models. The new flap also has better demarcation of necrosis area in this study.
麦克法伦皮瓣或背侧带蒂皮瓣已成为带蒂鼠皮瓣研究的标准模型，但它的可靠性受到质疑。过去，麦克法伦皮瓣可能存在混杂变量，因此采用了各种方法来调整这些变量。我们已经开发了一种新的模型来研究皮瓣坏死及其预防，该模型消除了这些混杂变量。
该皮瓣是麦克法伦皮瓣的重大改进，我们使用 3cm×9cm 的背侧皮瓣形成一个盲端带蒂管。使用数字摄影和计算机辅助分析来测量存活面积。将这种新皮瓣与标准的麦克法伦皮瓣（每组 n=25）进行比较。
新皮瓣（15.673cm²+SD3.37）的平均存活面积与麦克法伦皮瓣（18.904cm²+SD3.79）相当。其相对优势在于消除了移植物床对皮瓣的影响这一混杂变量，而不会显著降低皮瓣的存活面积。
提出了一种新的大鼠模型，可用于研究各种治疗方法对带蒂皮瓣的影响。与其他模型相比，该模型具有消除移植物床效应的优点，同时不会出现皮瓣和伤口感染或干燥的风险。在这项研究中，新皮瓣的坏死区域也有更好的分界。
