Department of Science & Technology Centre on Biomolecular Electronics, National Physical Laboratory, Dr K. S. Krishnan Marg, New Delhi-110012, India.
J Mol Recognit. 2010 Sep-Oct;23(5):472-9. doi: 10.1002/jmr.1014.
Polyaniline/carbon nanotubes composite (PANI-CNT) electrochemically deposited onto indium-tin-oxide (ITO) coated glass plate has been utilized for Neisseria gonorrhoeae detection by immobilizing 5'-amino-labeled Neisseria gonorrhoeae probe (aDNA) using glutaraldehyde as a cross-linker. PANI-CNT/ITO and aDNA-Glu-PANI-CNT/ITO electrodes have been characterized using scanning electron microscopy (SEM), Fourier Transform Infrared (FT-IR) spectroscopy, cyclic voltammetry (CV), and differential pulse voltammetry (DPV). This bioelectrode can be used to detect N. gonorrhoeae using methylene blue (MB) as redox indicator with response time of 60 s and stability of about 75 days when stored under refrigerated conditions. DPV studies reveal that this bioelectrode can detect complementary DNA concentration from 1 x 10(-6) M to 1 x 10(-17) M with detection limit of 1.2 x 10(-17) M. Further, this bioelectrode (aDNA-Glu-PANI-CNT/ITO) exhibits specificity toward N. gonorrhoeae species and shows negative response with non-Neisseria gonorrhoeae Neisseria species (NgNS) and other gram negative bacteria (GNB).
聚邻苯二胺/碳纳米管复合（PANI-CNT）电化学沉积在氧化铟锡（ITO）涂覆的玻璃板上，已被用于淋病奈瑟菌检测，方法是使用戊二醛作为交联剂固定 5'-氨基标记的淋病奈瑟菌探针（aDNA）。PANI-CNT/ITO 和 aDNA-Glu-PANI-CNT/ITO 电极已通过扫描电子显微镜（SEM）、傅里叶变换红外（FT-IR）光谱、循环伏安法（CV）和差分脉冲伏安法（DPV）进行了表征。该生物电极可以使用亚甲基蓝（MB）作为氧化还原指示剂来检测淋病奈瑟菌，响应时间为 60 秒，在冷藏条件下储存时稳定性约为 75 天。DPV 研究表明，该生物电极可以检测从 1×10^(-6) M 到 1×10^(-17) M 的互补 DNA 浓度，检测限为 1.2×10^(-17) M。此外，该生物电极（aDNA-Glu-PANI-CNT/ITO）对淋病奈瑟菌种具有特异性，并且对非淋病奈瑟菌淋病奈瑟菌（NgNS）和其他革兰氏阴性菌（GNB）表现出阴性反应。
