Department of Science and Technology Centre on Biomolecular Electronics, Biomedical Instrumentation Section, Materials Physics and Engineering Division, National Physical Laboratory, Dr. K. S. Krishnan Marg, New Delhi, India.
Electrophoresis. 2010 Nov;31(22):3754-62. doi: 10.1002/elps.201000321.
Polyaniline nanotube (PANI-NT) based films have been fabricated onto indium-tin-oxide (ITO) coated glass plates via electrophoretic technique. These PANI-NT/ITO electrodes have been utilized for covalent immobilization of cholesterol oxidase (ChOx) using glutaraldehyde (Glu) as cross-linker. Structural, morphological and electrochemical characterization of PANI-NT/ITO electrode and ChOx/Glu/PANI-NT/ITO bioelectrode have been done using FT-IR spectroscopy, SEM, electrochemical impedance spectroscopy and cyclic voltammetry techniques. Response studies of the ChOx/Glu/PANI-NT/ITO bioelectrode have been carried out using both linear sweep voltammetry and UV-Visible spectrophotometry. The results of the biosensing studies reveal that this bioelectrode can be used to detect cholesterol in wide detection range of 25-500 mg/dL with high sensitivity of 3.36 mA mg(-1) dL and fast response time of 30 s at pH 7.4. This bioelectrode exhibits very low value of Michaelis-Menten constant of 1.18 mM indicating enhanced interactions between cholesterol and ChOx immobilized onto this nanostructured PANI matrix.
通过电泳技术，将聚苯胺纳米管（PANI-NT）基膜制备到氧化铟锡（ITO）涂覆的玻璃板上。这些 PANI-NT/ITO 电极已被用于通过戊二醛（Glu）作为交联剂将胆固醇氧化酶（ChOx）共价固定化。使用傅里叶变换红外光谱、SEM、电化学阻抗谱和循环伏安法技术对 PANI-NT/ITO 电极和 ChOx/Glu/PANI-NT/ITO 生物电极的结构、形态和电化学特性进行了表征。使用线性扫描伏安法和紫外可见分光光度法对 ChOx/Glu/PANI-NT/ITO 生物电极的响应进行了研究。生物传感研究的结果表明，该生物电极可用于在 25-500mg/dL 的宽检测范围内检测胆固醇，具有 3.36 mA mg(-1) dL 的高灵敏度和 30s 的快速响应时间，在 pH7.4 下。该生物电极表现出非常低的米氏常数（1.18mM），表明胆固醇与固定在这种纳米结构 PANI 基质上的 ChOx 之间的相互作用增强。
