ImmunoTechnology Section, Vaccine Research Center, National Institutes of Health, Bethesda, MD 20892, USA.
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2010 Jul-Aug;2(4):334-48. doi: 10.1002/wnan.75.
Because of their unique fluorescence properties, quantum dots (QDs) represent a promising new technology in the realm of multicolor flow cytometry. Although commercial reagents and applications for the technology are still in the early phases of their development, the strategies and considerations necessary for successful use are becoming known. This article discusses the value of QDs in multicolor flow cytometry, introduces strategies to successfully incorporate QDs into routine use, and highlights emerging applications of the technology.
由于其独特的荧光特性,量子点 (QD) 在多色流式细胞术领域代表了一种很有前途的新技术。尽管该技术的商业试剂和应用仍处于早期开发阶段,但成功使用所需的策略和考虑因素正在逐渐明晰。本文讨论了 QD 在多色流式细胞术中的价值,介绍了成功将 QD 纳入常规使用的策略,并重点介绍了该技术的新兴应用。