Ma Xiao-Jing, Zhao Hong-Wei, Liu Gui-Feng, Ji Te, Zhang Zeng-Yan, Dai Bin
Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2009 Nov;29(11):2885-8.
Terahertz (THz) radiation lies between the infrared and microwave range of the electromagnetic spectrum. Absorption spectrum in the THz range provides rich information about structure and weak interactions of biomolecules. THz absorption spectra of D-(-)-ribose, D-glucose, alpha-lactose monohydrate and beta-lactose were measured by terahertz time-domain spectroscopy (THz-TDS) in the frequency range of 0.3-1.6 THz at room temperature. The experimental results show that different saccharides have distinct THz absorption features, which suggests that THz-TDS is highly sensitive to molecular structures and components. Quantitative analysis of the mixtures of two to four saccharides was studied by linear regression with relative error less than 7.2%. The reasons for the relative error were discussed. The results demonstrate that THz-TDS is a promising and efficient method for both qualitative and quantitative analyses for pharmaceutical identification and biomolecular detection.
太赫兹（THz）辐射位于电磁频谱的红外和微波范围之间。太赫兹范围内的吸收光谱提供了有关生物分子结构和弱相互作用的丰富信息。在室温下，通过太赫兹时域光谱（THz-TDS）在0.3-1.6太赫兹的频率范围内测量了D-（-）-核糖、D-葡萄糖、α-乳糖一水合物和β-乳糖的太赫兹吸收光谱。实验结果表明，不同的糖类具有明显的太赫兹吸收特征，这表明THz-TDS对分子结构和成分高度敏感。通过线性回归研究了两种至四种糖类混合物的定量分析，相对误差小于7.2%。讨论了相对误差产生的原因。结果表明，THz-TDS是一种用于药物鉴定和生物分子检测的定性和定量分析的有前景且高效的方法。
