Yin Wen-Xuan, Liu Yu-Feng, Zhao Ying, Bai Song-Yan, Zhu Wei-Wei, Weng Shi-Fu, Gao Qing-Yu, Wu Jin-Guang
College of Chemical Engineering, China University of Mining Technology and Engineering, Xuzhou 221008, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2009 Nov;29(11):3101-5.
In the simulation experiments in vitro of the formation of gallstone, adenosine-triphosphate(ATP)-Co(2+)-deoxycholic acid(DC) gel system was chosen to study the periodic precipitation progress. The effect of ATP on the Co(2+)-DC gel system was also determined, and the structure of the periodic precipitation formed was characterized by FTIR. The results show that the patterns formed in the systems with ATP are different, ATP affected the rate and structure of precipitation through its variable participation in the metal coordination complexes as judged by the phosphate P=O bands and the deoxycholate COO- symmetric and asymmetric vibration bands as measured by FTIR Theses spectroscopic differences were correlated with color and pattern differences in the precipitates. ATP has a more remarkable function than AMP to the modes of patterns, meanwhile the system patterns transform from fractal to periodic precipitation. There is a complex interaction among ATP, sodium deoxycholic and Co2+ with a transparent crystal produced. The crystal is deoxycholic acid and the periodic precipitation is composed of ATP and DC covalent to Co2+. These results indicate that stone formation and remodeling is a dynamic, nonlinear progress. Much of the precipitate, as judged by local differences in composition, is not in equilibrium with the general gel environment. The authors conclude that the formation of gallstone features complex and nonlinear chemical character, in which nucleotides as living material play a very important role.
在胆结石形成的体外模拟实验中，选用三磷酸腺苷（ATP）-钴（2+）-脱氧胆酸（DC）凝胶体系来研究周期性沉淀过程。还测定了ATP对钴（2+）-DC凝胶体系的影响，并用傅里叶变换红外光谱（FTIR）对形成的周期性沉淀结构进行了表征。结果表明，含ATP体系中形成的图案不同，通过FTIR测量的磷酸根P=O带以及脱氧胆酸盐COO-对称和不对称振动带判断，ATP通过其在金属配位络合物中的可变参与影响沉淀速率和结构。这些光谱差异与沉淀物的颜色和图案差异相关。ATP对图案模式的作用比AMP更显著，同时体系图案从分形转变为周期性沉淀。ATP、脱氧胆酸钠和钴2+之间存在复杂的相互作用并产生了透明晶体。该晶体为脱氧胆酸，周期性沉淀由与钴共价结合的ATP和DC组成。这些结果表明结石形成和重塑是一个动态的、非线性过程。从组成的局部差异判断，大部分沉淀物与一般凝胶环境不平衡。作者得出结论，胆结石的形成具有复杂的非线性化学特征，其中作为生物物质的核苷酸起着非常重要的作用。
