Laboratory for High Performance Ceramics, Empa, Swiss Federal Laboratories for Materials Testing and Research, Duebendorf, Switzerland.
Institute for Ceramics, Glass and Construction Materials, Technical University Bergakademie Freiberg, Freiberg, Germany.
J Appl Microbiol. 2010 Aug;109(2):388-397. doi: 10.1111/j.1365-2672.2010.04663.x. Epub 2010 Jan 22.
Viruses as well as other (bio-)colloids possess a pH-dependent surface charge in polar media such as water. This electrostatic charge determines the mobility of the soft particle in an electric field and thus governs its colloidal behaviour which plays a major role in virus sorption processes. The pH value at which the net surface charge switches its sign is referred to as the isoelectric point (abbreviations: pI or IEP) and is a characteristic parameter of the virion in equilibrium with its environmental water chemistry. Here, we review the IEP measurements of viruses that replicate in hosts of kingdom plantae, bacteria and animalia. IEPs of viruses are found in pH range from 1.9 to 8.4; most frequently, they are measured in a band of 3.5 < IEP < 7. However, the data appear to be scattered widely within single virus species. This discrepancy is discussed and should be considered when IEP values are used to account for virus sorption processes.
病毒以及其他（生物）胶体在极性介质（如水中）中具有 pH 依赖性表面电荷。这种静电荷决定了软颗粒在电场中的迁移率，从而控制其胶体行为，这在病毒吸附过程中起着重要作用。净表面电荷符号发生变化的 pH 值称为等电点（缩写：pI 或 IEP），是与环境水化学平衡的病毒粒子的特征参数。在这里，我们回顾了在植物界、细菌和动物宿主中复制的病毒的 IEP 测量。病毒的 IEP 值在 pH 值 1.9 到 8.4 之间；最常见的是，它们在 3.5<IEP<7 的范围内进行测量。然而，数据似乎在单个病毒种内广泛分散。当使用 IEP 值来解释病毒吸附过程时，应该讨论并考虑这种差异。
