Professional Graduate School of Oriental Medicine, Wonkwang University, Chonbuk, Republic of Korea.
Phytother Res. 2010 Jun;24 Suppl 2:S214-9. doi: 10.1002/ptr.3099.
Inflammatory mediators such as tumor necrosis factor-alpha (TNF-alpha) enhance binding of low-density lipoprotein to endothelium and upregulate the expression of endothelial leukocyte adhesion molecules during atherogenesis. The present study examined the effect of ethanol extract of Gardenia jasminoides (EGJ) on vascular inflammation in primary cultured human umbilical vein endothelial cells (HUVEC). TNF-alpha-induced the expression of vascular cell adhesion molecule-1 (VCAM-1) and endothelial cell-selectin (E-selectin) expression was inhibited in HUVEC pretreated with EGJ. In a functional study, EGJ dose-dependently attenuated adhesion of HL-60 monocytes to endothelial monolayers. A further analysis indicated that EGJ attenuated TNF-alpha-induced nuclear p65 nuclear factor-kappa B (NF-kappaB) translocation, suggesting that EGJ primarily affects the TNF-alpha-induced NF-kappaB signaling pathway. Taken together, we provided here the first evidence showing that EGJ is able to inhibit TNF-alpha-induced NF-kappaB activation, adhesion molecule expression, and monocyte-endothelial interaction, suggesting an anti-inflammatory role of EGJ, which may be useful in preventing vascular diseases, such as atherosclerosis.
炎症介质，如肿瘤坏死因子-α（TNF-α），在动脉粥样硬化形成过程中增强了低密度脂蛋白与内皮的结合，并上调了内皮白细胞黏附分子的表达。本研究探讨了栀子（EGJ）乙醇提取物对原代培养的人脐静脉内皮细胞（HUVEC）血管炎症的影响。TNF-α诱导血管细胞黏附分子-1（VCAM-1）的表达，而 EGJ 预处理可抑制内皮细胞选择素（E-selectin）的表达。在功能研究中，EGJ 呈剂量依赖性地减弱 HL-60 单核细胞与内皮单层的黏附。进一步的分析表明，EGJ 可减弱 TNF-α诱导的核 p65 核因子-κB（NF-κB）易位，提示 EGJ 主要影响 TNF-α诱导的 NF-κB 信号通路。综上所述，我们首次提供了证据表明，EGJ 能够抑制 TNF-α诱导的 NF-κB 激活、黏附分子表达和单核细胞-内皮细胞相互作用，提示 EGJ 具有抗炎作用，可能有助于预防血管疾病，如动脉粥样硬化。
