Chen Jianrong, Tchivelekete Gabriel Mbuta, Zhou Xinzhi, Tang Weizhuo, Liu Fang, Liu Minzhuo, Zhao Chenxi, Shu Xinhua, Zeng Zhihong
College of Biological and Environmental Engineering, Changsha University, Changsha, Hunan 410022, P.R. China.
Department of Biological and Biomedical Sciences, Glasgow Caledonian University, Glasgow, G4 0BA, UK.
Exp Ther Med. 2021 Jul;22(1):700. doi: 10.3892/etm.2021.10132. Epub 2021 May 2.
Age-related macular degeneration (AMD) is the most common cause of visual impairment in developed countries. Inflammation serves a critical role in the pathogenesis of AMD. is found in several regions of China and is traditionally used as an organic yellow dye but has also been widely used as a therapeutic agent in numerous diseases, including inflammation, depression, hepatic and vascular disorders, which may reflect the variability of functional compounds that are present in extracts (GJE). To investigate the therapeutic potential of GJE for AMD, ARPE-19 cells were treated with lipopolysaccharide (LPS) or LPS plus GJE. GJE significantly decreased LPS-induced expression of proinflammatory cytokines, including IL-1β, IL-6 and TNF-α. In the study, GJE inhibited CuSO4-induced migration of primitive macrophages to the lateral line in zebrafish embryos. GJE also attenuated expression of cytokines (IL-1β, IL-6 and TNF-α), NFKB activating protein () and TLR4 in ARPE-19 cells. The results of the present study demonstrated the anti-inflammatory potential of GJE and , and suggested GJE as a therapeutic candidate for AMD.
年龄相关性黄斑变性（AMD）是发达国家视力损害的最常见原因。炎症在AMD的发病机制中起关键作用。在中国的几个地区被发现，传统上用作有机黄色染料，但也被广泛用作多种疾病的治疗剂，包括炎症、抑郁症、肝脏和血管疾病，这可能反映了提取物（GJE）中存在的功能化合物的变异性。为了研究GJE对AMD的治疗潜力，用脂多糖（LPS）或LPS加GJE处理ARPE - 19细胞。GJE显著降低LPS诱导的促炎细胞因子的表达，包括IL - 1β、IL - 6和TNF -α。在该研究中，GJE抑制了硫酸铜诱导的原始巨噬细胞向斑马鱼胚胎侧线的迁移。GJE还减弱了ARPE - 19细胞中细胞因子（IL - 1β、IL - 6和TNF -α）、NFKB激活蛋白（）和TLR4的表达。本研究结果证明了GJE的抗炎潜力，并表明GJE作为AMD的治疗候选物。
