[A组和B组人呼吸道合胞病毒N蛋白的基因特征分析]

[Genetic characterization of the N protein of subgroups A and B human respiratory syncytial viruses].

作者信息

Zhang Yan, Wang Hui-ling, Xie Zheng-de, Kong Xiao-hui, Liu Chun-yan, Shen Kun-ling, Xu Wen-bo

机构信息

National Institute for Viral Disease Control and Prevention, China Center for Disease Control and Prevention, Beijing 100050, China.

出版信息

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2009 Apr;23(2):115-7.

PMID:20104752

Abstract

OBJECTIVE

To clarify the genetic characteristics of N protein coding region of HRSV isolates from Beijing and GenBank downloaded sequences.

METHODS

Reverse transciption polymerase chain reaction (RT-PCR) was performed to amplify the N protein gene of 2 A and 2 B subgroups HRSV isolates from Beijing in the year 2004. The RT-PCR products were sequenced for N protein coding region. The sequences of N protein coding region of 4 Beijing isolates and those downloaded from GenBank were compared and analyzed.

RESULTS

The differences in number of nucleotide and deduced amino acid between 2 A Beijing 2004 isolates and prototype strain Long were 36-40 (3.1%-3.4%) and 4 (1.0%). The differences in number of nucleotide and deduced amino acid between 2 B Beijing 2004 isolates and prototype strain CH18537 were 17 (1.4%) and 1 (0.3%). The differences in number of nucleotide and deduced amino acid were 3-172 (0.25%-14.63%) and 0-18 (0-4.6%) respectively between 4 Beijing 2004 isolates and GenBank sequences.

CONCLUSION

N gene is the highly conservative gene in the HRSV genome. The variation between A and B subgroups were widely distributed in the entire gene of N protein, while the variation within the A or B subgroups HRSV was considerably lower.

摘要

目的

阐明北京地区人呼吸道合胞病毒（HRSV）分离株N蛋白编码区的基因特征以及从GenBank下载序列的基因特征。

方法

采用逆转录聚合酶链反应（RT-PCR）扩增2004年北京地区2个A亚组和2个B亚组HRSV分离株的N蛋白基因。对RT-PCR产物的N蛋白编码区进行测序。将4株北京分离株N蛋白编码区序列与从GenBank下载的序列进行比较分析。

结果

2004年北京地区2个A亚组分离株与原型株Long在核苷酸数量和推导氨基酸数量上的差异分别为36 - 40（3.1% - 3.4%）和4（1.0%）。2004年北京地区2个B亚组分离株与原型株CH18537在核苷酸数量和推导氨基酸数量上的差异分别为17（1.4%）和1（0.3%）。4株2004年北京分离株与GenBank序列在核苷酸数量和推导氨基酸数量上的差异分别为3 - 172（0.25% - 14.63%）和0 - 18（0 - 4.6%）。