Zhang Lei, Zhao Rui, Zhong Ru-gang, Zhou Ling, Li De-chang, Wu Yong-ning, Zeng Yi
Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing 100052, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2009 Apr;23(2):121-3.
To improve the protocol of BALB/c 3T3 cell transformation assay, and apply it to the cocarcinogenesis study.
Appropriate serum concentration, culture media and method of administration were selected by testing their effects on the growth and transformation of BALB/c 3T3 cells. The co-carcinogenic activity between diethylnitrosamine (DEN) and microcystin-LR (MC-LR) or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) were examined using the improved cell transformation assay. The malignant characteristics of transformed cells were verified by neoplasia in SCID mice.
There were strong co-carcinogenic activity between DEN and TCDD. On the contrary, although MC-LR has strong ability to induce cell transformation, the effect was markely inhibited by DEN. The transformed cells show some malignant characteristics.
The improved BALB/c 3T3 cell transformation assay is reliable and time-saving, and can be efficiently used in the study of cocarcinogenesis.
改进BALB/c 3T3细胞转化试验方案，并将其应用于协同致癌研究。
通过检测其对BALB/c 3T3细胞生长和转化的影响，选择合适的血清浓度、培养基和给药方法。使用改进的细胞转化试验检测二乙基亚硝胺（DEN）与微囊藻毒素-LR（MC-LR）或2,3,7,8-四氯二苯并-对-二恶英（TCDD）之间的协同致癌活性。通过SCID小鼠肿瘤形成验证转化细胞的恶性特征。
DEN与TCDD之间存在很强的协同致癌活性。相反，尽管MC-LR具有很强的诱导细胞转化能力，但该作用被DEN显著抑制。转化细胞表现出一些恶性特征。
改进后的BALB/c 3T3细胞转化试验可靠且省时，可有效用于协同致癌研究。
