Atchison M, Chu C, Kakunaga T, Van Duuren B L
J Natl Cancer Inst. 1982 Aug;69(2):503-8.
This study was performed for the detection of cocarcinogens by transformation in vitro with the use of a subclone of a Balb/3T3 cell line. Dose response, cytotoxicity, and transformation studies were done with the use of an indirect-acting carcinogen, benzo[a]pyrene (B[a]P), a direct-acting alkylating carcinogen, beta-propiolactone (BPL), and the mouse skin cocarcinogen catechol. The rate of transformation was notably higher in groups treated with B[a]P and catechol or BPL and catechol than in groups treated with either B[a]P or BPL. Catechol alone did not induce any transformation. All the cells isolated from the transformed foci showed characteristics of malignantly transformed cells, such as anchorage-independent growth. Thus chemical cocarcinogenesis was accomplished in vitro similar to that accomplished in in vivo studies reported earlier on mouse skin.
本研究利用Balb/3T3细胞系的一个亚克隆，通过体外转化来检测共致癌物。使用间接作用致癌物苯并[a]芘（B[a]P）、直接作用烷基化致癌物β-丙内酯（BPL）以及小鼠皮肤共致癌物儿茶酚进行剂量反应、细胞毒性和转化研究。用B[a]P与儿茶酚或BPL与儿茶酚处理的组中，转化速率显著高于用B[a]P或BPL单独处理的组。单独的儿茶酚未诱导任何转化。从转化灶分离出的所有细胞均表现出恶性转化细胞的特征，如不依赖贴壁生长。因此，体外化学共致癌作用的实现方式与先前报道的小鼠皮肤体内研究相似。
