[诺如病毒酶联免疫吸附测定试剂盒的效果研究]
[Study on effect of enzyme linked immunosorbent assay kits of norovirus].
作者信息
Zhang Qing, Jin Miao, Cui Shu-Xian, Liu Na, Li Lei, Duan Zhao-Jun
机构信息
Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China.
出版信息
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2009 Jun;23(3):227-8.
OBJECTIVE
Effects of RIDASCREEN Norovirus (C 1401) 3rd Generation kit (R-biopham AG, darmstadt, Germany) and IDEIA NLV kit (DAKOCytomation., Ely, UK) were compared for detecting human norovirus (HuNV) in fecal sample.
METHODS
The performance of the ELISA was compared with that of the reverse transcription polymerase chain reaction (RT-PCR) by testing a panel of 308 fecal samples collected from patients involved in outbreaks of gastroenteritis in Chang Chun and Guang Zhou. Gene sequencing was performed to positive samples tested by RT-PCR to determine genotype compared with standard sequences.
RESULTS
RT-PCR is gold standard, RIDASCREEN Norovirus (C 1401) 3rd Generation kit had a high sensitivity of 96.10% but a specificity of 93.51%, and Dako kit had a low sensitivity of 95.83% but a high specificity of 95.76%.
CONCLUSION
RIDASCREEN Norovirus (C 1401) 3rd Generation kit is more Satisfactory for a preliminary screening.
目的
比较RIDASCREEN诺如病毒（C 1401）第三代检测试剂盒（德国达姆施塔特R-生物制药公司）和IDEIA NLV检测试剂盒（英国伊利DAKOCytomation公司）检测粪便样本中人类诺如病毒（HuNV）的效果。
方法
通过检测从长春和广州参与胃肠炎暴发的患者中收集的308份粪便样本，将酶联免疫吸附测定（ELISA）的性能与逆转录聚合酶链反应（RT-PCR）的性能进行比较。对经RT-PCR检测呈阳性的样本进行基因测序，以确定与标准序列相比的基因型。
结果
RT-PCR是金标准，RIDASCREEN诺如病毒（C 1401）第三代检测试剂盒灵敏度高，为96.10%，但特异性为93.51%，达科试剂盒灵敏度低，为95.83%，但特异性高，为95.76%。
结论
RIDASCREEN诺如病毒（C 1401）第三代检测试剂盒用于初步筛查更令人满意。
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