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免疫层析法和商用酶联免疫吸附测定法用于粪便样本中诺如病毒抗原快速检测的评估。

Evaluation of immunochromatography and commercial enzyme-linked immunosorbent assay for rapid detection of norovirus antigen in stool samples.

作者信息

Khamrin Pattara, Nguyen Tuan Anh, Phan Tung Gia, Satou Kenji, Masuoka Yuichi, Okitsu Shoko, Maneekarn Niwat, Nishio Osamu, Ushijima Hiroshi

机构信息

Department of Developmental Medical Sciences, Institute of International Health, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan.

出版信息

J Virol Methods. 2008 Feb;147(2):360-3. doi: 10.1016/j.jviromet.2007.09.007. Epub 2007 Oct 23.

DOI:10.1016/j.jviromet.2007.09.007

PMID:17959257

Abstract

The efficiency of immunochromatography and commercial enzyme-linked immunosorbent assay (ELISA) kit (Denka Seiken Co. Ltd., Tokyo, Japan) were evaluated for rapid detection of norovirus (NoV) from stool specimens. A total of 503 stool specimens collected from infants and young children who suffered from acute gastroenteritis were tested for NoV by the NoV-immunochromatography kit, Denka ELISA kit, and by a monoplex RT-PCR method. The NoV-immunochromatography revealed 78.9% sensitivity, 96.4% specificity, and 92.4% efficiency with the monoplex RT-PCR method. The Denka ELISA kit had a sensitivity of 90.4%, specificity of 96.4%, and an efficiency level of 95%. The findings indicate that the newly developed NoV-immunochromatography kit provides the specificity equal to that of the Denka ELISA kit, even through the sensitivity of detection was lower. However, the advantage of the NoV-immunochromatography kit is less time consuming and simpler. The data show that both the Denka ELISA and the NoV-immunochromatography kits may be used as an alternative method for screening of NoV in stool samples.

摘要

对免疫层析法和商用酶联免疫吸附测定（ELISA）试剂盒（日本东京电和化成工业株式会社）从粪便标本中快速检测诺如病毒（NoV）的效率进行了评估。采用诺如病毒免疫层析试剂盒、电和ELISA试剂盒以及单重逆转录聚合酶链反应（RT-PCR）方法，对503份从患有急性肠胃炎的婴幼儿收集的粪便标本进行了诺如病毒检测。诺如病毒免疫层析法显示，与单重RT-PCR方法相比，其灵敏度为78.9%，特异性为96.4%，效率为92.4%。电和ELISA试剂盒的灵敏度为90.4%，特异性为96.4%，效率水平为95%。研究结果表明，新开发的诺如病毒免疫层析试剂盒虽然检测灵敏度较低，但其特异性与电和ELISA试剂盒相当。然而，诺如病毒免疫层析试剂盒的优点是耗时更少且操作更简单。数据显示，电和ELISA试剂盒以及诺如病毒免疫层析试剂盒均可作为粪便样本中诺如病毒筛查的替代方法。