Flø R W, Naess A, Lund-Johansen F, Maehle B O, Sjursen H, Lehmann V, Solberg C O
Medical Department B, Haukeland Hospital, University of Bergen, Norway.
J Immunol Methods. 1991 Mar 1;137(1):89-94. doi: 10.1016/0022-1759(91)90397-x.
We have developed a standardized procedure for the isolation of monocytes from peripheral blood by negative selection using magnetic polymer particles coated with monoclonal antibodies against T and B lymphocytes. The average purity of the monocyte suspension was 85%, and monocyte recovery was 72% from Ficoll-Hypaque gradient separated mononuclear cells and 32% from whole blood. In a lucigenin enhanced chemiluminescence assay there was no significant difference between cells separated immunomagnetically and those separated on a gradient. Nor did electron microscopy show any significant difference in morphology between such monocytes. Negative selection using magnetic polymer particles is an efficient method for the separation of monocytes with intact morphology and function as measured by chemiluminescence.
我们已开发出一种标准化程序，通过使用包被有抗T和B淋巴细胞单克隆抗体的磁性聚合物颗粒进行阴性选择，从外周血中分离单核细胞。单核细胞悬液的平均纯度为85%，单核细胞回收率在Ficoll-Hypaque梯度分离的单个核细胞中为72%，在全血中为32%。在光泽精增强化学发光测定中，免疫磁珠分离的细胞与梯度分离的细胞之间没有显著差异。电子显微镜检查也未显示此类单核细胞在形态上有任何显著差异。使用磁性聚合物颗粒进行阴性选择是一种有效的方法，可分离出形态和功能完整（通过化学发光测定）的单核细胞。
