Sanderson R J, Shepperdson R T, Vatter A E, Talmage D W
J Immunol. 1977 Apr;118(4):1409-14.
Monocytes were isolated by counterflow contrifugation from human peripheral mononuclear cells obtained from whole blood by the Ficoll-Hypaque technique. Examination of volume spectra of the monocytes showed a bimodal distribution with peaks at 330 and 370 micron3. The upper peak contained 61.4 +/- 3.3% of the total monocyte population, whereas the remainder were in the lower peak which overlaps the lymphocyte spectrum. The purity of the monocyte preparations from six normal adult donors, identified by peroxisomes observed by electron microscopy, was 90.4 +/- 1.6%.
通过Ficoll-Hypaque技术从全血中获得人外周血单个核细胞，然后采用逆流离心法分离单核细胞。对单核细胞的体积光谱进行检查，结果显示呈双峰分布，峰值分别在330和370立方微米处。较高的峰值包含了61.4±3.3%的单核细胞总数，而其余的则在较低的峰值中，该峰值与淋巴细胞光谱重叠。通过电子显微镜观察过氧化物酶体鉴定，来自6名正常成年供体的单核细胞制剂纯度为90.4±1.6%。
