Wicher V, Zabek J, Wicher K
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201-0509.
J Infect Dis. 1991 Apr;163(4):830-6.
Molecular analysis of the humoral response to pathogen-specific polypeptides was done using sera from patients at different stages of syphilis and sera from Treponema pallidum-infected rabbits and guinea pigs collected at various times after infection. The sera were examined by ELISA, fluorescent treponemal antibody absorption test (FTA-ABS), and immunoblot before and after sequential adsorption with cyanogen bromide-activated Sepharose 4B coupled individually to five sonicated nonpathogenic treponemes. Guinea pig antisera were also adsorbed with rabbit proteins. After adsorption, sera from all three species reacted neither by ELISA nor by immunoblot with nonpathogenic treponemes, nor did guinea pig sera react with rabbit proteins. Regardless of the species of treponema used for initial adsorption, none by itself could effectively remove all cross-reactivities. Nine pathogen-specific polypeptides (15, 17, 33, 37, 39, 43, 45, 47, 97 kDa), eight (15-47 kDa) known as integral membrane or endoflagellar components, were commonly recognized by the tree species when chancre immunity developed. The substantial reduction in immune reactivity after adsorption suggests that most of these peptides consist of specific and common treponemal epitopes. Throughout the various stages of the human disease, pathogen-specific antibodies were exclusively of the IgG isotype.
利用梅毒不同阶段患者的血清以及梅毒螺旋体感染的兔子和豚鼠在感染后不同时间收集的血清，对针对病原体特异性多肽的体液反应进行了分子分析。在用分别偶联五种超声处理的非致病性梅毒螺旋体的溴化氰活化琼脂糖4B进行连续吸附之前和之后，通过酶联免疫吸附测定（ELISA）、荧光密螺旋体抗体吸收试验（FTA-ABS）和免疫印迹法检测血清。豚鼠抗血清也用兔蛋白进行吸附。吸附后，所有三个物种的血清通过ELISA和免疫印迹法均不与非致病性梅毒螺旋体发生反应，豚鼠血清也不与兔蛋白发生反应。无论用于初始吸附的梅毒螺旋体种类如何，其本身均不能有效去除所有交叉反应性。当硬下疳免疫力形成时，三种物种共同识别出九种病原体特异性多肽（15、17、33、37、39、43、45、47、97 kDa），其中八种（15 - 47 kDa）被认为是整合膜或内鞭毛成分。吸附后免疫反应性的显著降低表明，这些多肽中的大多数由特异性和常见的梅毒螺旋体表位组成。在人类疾病的各个阶段，病原体特异性抗体均仅为IgG同种型。
