Wicher K, Jakubowski A, Wicher V
Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany 12201.
Clin Exp Immunol. 1987 Aug;69(2):263-70.
Young male inbred strain 2 guinea pigs were infected intradermally with 8 X 10(7) Treponema pallidum extracted from a rabbit orchitis, and 5 months later reinfected with 10(7) T. pallidum. Ninety percent of the animals developed symptomatic lesions after initial infection but none on challenge. Immunoblotting of sera obtained at intervals after infection or reinfection showed antibodies against T. pallidum antigen (TP), nonpathogenic treponemes--T. phagedenis biotype Reiter (TR), T. refringens strain Noguchi (TN), and T. vincentii (TV)--as well as normal rabbit serum (NRS) and normal rabbit testes extract (NRT). Antibodies reacting with TP were detected as early as 17 days (five polypeptides) and steadily rose (at 3 months 17 polypeptides were seen). Cross-reacting antibodies to TR, TN, TV, or rabbit proteins decreased within 3 to 5 months. After reinfection, the antibodies to NRS increased more sharply than the anti-treponemal antibodies. Adsorption with TR and NRS of sera obtained after infection or reinfection produced a reduction of antibodies to TP by 75-87%.
将从兔睾丸炎中提取的8×10⁷梅毒螺旋体皮内接种于年轻的雄性近交系2豚鼠，5个月后再用10⁷梅毒螺旋体进行再感染。90%的动物在初次感染后出现有症状的病变，但再次感染时均未出现。对感染或再感染后不同时间采集的血清进行免疫印迹分析，结果显示存在针对梅毒螺旋体抗原（TP）、非致病性密螺旋体——溃蚀性密螺旋体生物变种Reiter（TR）、致密密螺旋体诺古奇菌株（TN）和奋森密螺旋体（TV）的抗体，以及正常兔血清（NRS）和正常兔睾丸提取物（NRT）。早在17天就能检测到与TP反应的抗体（五条多肽），且抗体水平持续上升（3个月时可见17条多肽）。针对TR、TN、TV或兔蛋白的交叉反应抗体在3至5个月内减少。再次感染后，针对NRS的抗体比抗密螺旋体抗体升高得更明显。用TR和NRS吸附感染或再感染后获得的血清，可使针对TP的抗体减少75%至87%。
