水通道蛋白在人乳腺癌中的鉴定及其在乳腺癌发生中的作用。

Aquaporins in human breast cancer: identification and involvement in carcinogenesis of breast cancer.

机构信息

State Key Laboratory of Reproductive Medicine, Nanjing Maternity and Child Health Care Hospital Affiliated to Nanjing Medical University, Nanjing, China.

出版信息

J Surg Oncol. 2012 Sep 1;106(3):267-72. doi: 10.1002/jso.22155. Epub 2011 Nov 21.

DOI:10.1002/jso.22155

PMID:22105864

Abstract

BACKGROUND AND OBJECTIVES

Aquaporins (AQPs) play important roles in water and glycerol transport. Recently, the role of AQPs in human carcinogenesis has become an area of great interest. However, little is known about the function of AQPs in human breast cancer. The aim of this study was to investigate the expression profile of AQPs in human breast cancer and its significance.

METHODS

In this study, we screened the expression profile of AQP0-12 in breast cancer tissues and corresponding normal tissues by RT-PCR, Western blotting and immunohistochemistry.

RESULTS

AQP1, 3-5, and 10-12 were expressed in human breast cancer and/or normal breast tissues, and AQP1 and 3-5 exhibited differential expression. AQP1 was expressed in cell membranes and its expression was higher in cancer than that in normal tissues. AQP4 was expressed in the cell membrane and cytoplasm and was detected markedly stronger in normal than in cancer tissues. AQP5 was expressed mainly in cell membranes in carcinoma tissues, but was almost absent in normal breast tissues. Expression of AQP5 was associated with cellular differentiation, lymph node invasion, and clinicopathological staging.

CONCLUSIONS

These observations suggested that several subtypes of the AQP family play a role in human breast carcinogenesis.

摘要

背景与目的

水通道蛋白（AQP）在水和甘油运输中发挥重要作用。最近，AQP 在人类癌症发生中的作用成为研究热点。然而，AQP 在人类乳腺癌中的功能知之甚少。本研究旨在探讨 AQP 在人乳腺癌中的表达谱及其意义。

方法

本研究通过 RT-PCR、Western blot 和免疫组织化学方法筛选了 AQP0-12 在乳腺癌组织和相应正常组织中的表达谱。

结果

AQP1、3-5 和 10-12 在人乳腺癌和/或正常乳腺组织中表达，AQP1 和 3-5 表现出差异表达。AQP1 表达于细胞膜，在癌组织中的表达高于正常组织。AQP4 表达于细胞膜和细胞质，在正常组织中表达明显强于癌组织。AQP5 主要表达于癌组织的细胞膜，但在正常乳腺组织中几乎不存在。AQP5 的表达与细胞分化、淋巴结浸润和临床病理分期有关。