Polishchuk A S, Korotkoruchko V P
Ukr Biokhim Zh. 1977 Nov-Dec;49(6):66-70.
Cytoplasmic RNA of the rat regenerating liver and liver of rats with hepatoma PC-1 is separated into five fractions on the column of kieselgur with methylated albumin (MAK). Studies in the nucleotide composition showed that certain fractions of cytoplasm RNA are ribosomal and messenger RNA. The highest amount of radioactive precursor is incorporated into messenger RNA of the rat regenerating liver and liver of the rats with hepatoma PC-1 which is eluated from the column by 0.2% solution of sodium dodecyl sulphate at 37 degrees C (third fraction). Actinomycin D in doses inhibiting appearance of characteristic proteins in the blood serum of rats with the regenerating liver and heaptoma PC-1 blocks almost completely biosynthesis of ribosomal RNA eluated from the MAK column in the gradient of sodium chloride (second fraction), and that of messenger RNA, eluated by sodium dodecyl-sulphate at 37 degrees C (third fraction).
用甲基化白蛋白（MAK）硅胶柱对大鼠再生肝和肝癌PC - 1大鼠肝脏的细胞质RNA进行分离，可得到五个组分。对核苷酸组成的研究表明，细胞质RNA的某些组分是核糖体RNA和信使RNA。放射性前体的最高掺入量出现在大鼠再生肝和肝癌PC - 1大鼠肝脏的信使RNA中，该信使RNA在37℃下用0.2%十二烷基硫酸钠溶液从柱上洗脱（第三组分）。剂量能抑制再生肝和肝癌PC - 1大鼠血清中特征性蛋白质出现的放线菌素D，几乎完全阻断了在氯化钠梯度中从MAK柱上洗脱的核糖体RNA（第二组分）以及在37℃下用十二烷基硫酸钠洗脱的信使RNA（第三组分）的生物合成。
