Polishchuk A S, Korotkoruchko V P
Ukr Biokhim Zh. 1977 Jul-Aug;49(4):80-4.
Total nuclear RNA was isolated under conditions of actinomycin D blocking ion-exchange chromatography on kieselguhr columns with methylated albumin detected differences in transpiration of rat liver nuclear RNA with intensive normal and malignant growth. Actinomycin D in doses blocking the appearance of peculiar proteins in the blood serum of rats with the regenerating liver and RS-1 hepatoma produces a different effect on the biosynthesis of nuclear DNA-like RNA of the rat liver. 24h after a partial hepatectomy the antibiotic inhibits considerably the biosynthesis of nuclear DNA-like RNA which is eluated during chromatographying with 0.2% solution of sodium dodecyl sulphate at 70 degrees C. With RS-1 hepatoma the actinomycin D effect is most pronounced with respect to nuclear DNA-like RNA of rats with a tumour which is washed off from the column with a 0.2% solution of sodium dodecyl sulphate at 37 degrees C.
在放线菌素D阻断的条件下，从大鼠肝脏细胞核中分离出总核RNA。采用离子交换色谱法，在硅藻土柱上用甲基化白蛋白检测大鼠肝脏核RNA在正常生长和恶性生长情况下蒸腾作用的差异。放线菌素D的剂量能够阻断再生肝和RS-1肝癌大鼠血清中特殊蛋白质的出现，对大鼠肝脏核DNA样RNA的生物合成产生不同影响。部分肝切除术后24小时，该抗生素显著抑制核DNA样RNA的生物合成，这种RNA在70℃下用0.2%十二烷基硫酸钠溶液进行色谱分析时被洗脱。对于RS-1肝癌，放线菌素D对患有肿瘤的大鼠的核DNA样RNA的作用最为明显，该RNA在37℃下用0.2%十二烷基硫酸钠溶液从柱上洗脱下来。
