泰国健康个体粪便标本中产 CTX-M 型β-内酰胺酶肠杆菌科的高流行率。
High prevalence of CTX-M beta-lactamase-producing Enterobacteriaceae in stool specimens obtained from healthy individuals in Thailand.
机构信息
Department of Bioinformatics, Osaka University Graduate School of Medicine, Osaka, Japan.
出版信息
J Antimicrob Chemother. 2010 Apr;65(4):666-8. doi: 10.1093/jac/dkq008. Epub 2010 Jan 27.
OBJECTIVES
To determine the prevalence of CTX-M beta-lactamase-producing Enterobacteriaceae in stool specimens obtained from healthy individuals in a rural area of Thailand.
METHODS
Bacteria in stool specimens were screened for extended-spectrum beta-lactamase (ESBL) production on McConkey agar with cefotaxime and confirmed by the double-disc synergy test. Genetic detection and genotyping of CTX-M-type ESBL was performed by PCR with bacterial DNA extracted from isolates.
RESULTS
A markedly high number (82 of 141, 58.2%) of the specimens showed the presence of CTX-M beta-lactamase-producing Enterobacteriaceae, as confirmed by both phenotypic and genetic examinations. The majority of the CTX-M beta-lactamase-producing bacteria were Escherichia coli (85.1%).
CONCLUSIONS
The study revealed the wide dissemination of CTX-M beta-lactamase-producing Enterobacteriaceae in the healthy population.
目的
在泰国农村地区，从健康个体的粪便标本中确定 CTX-M 型β-内酰胺酶产生的肠杆菌科的流行率。
方法
在含有头孢噻肟的 McConkey 琼脂上筛选粪便标本中的产超广谱β-内酰胺酶（ESBL），并通过双碟协同试验进行确认。通过从分离物中提取细菌 DNA，使用 PCR 进行 CTX-M 型 ESBL 的基因检测和基因分型。
结果
通过表型和基因检查均证实，明显有大量（141 份标本中有 82 份，58.2%）标本存在 CTX-M 型β-内酰胺酶产生的肠杆菌科。大多数产 CTX-M 型β-内酰胺酶的细菌为大肠杆菌（85.1%）。
结论
该研究揭示了 CTX-M 型β-内酰胺酶产生的肠杆菌科在健康人群中的广泛传播。
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