Institute of Molecular Biology, Academia Sinica, Taipei 115; Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu 300.
Institute of Molecular Biology, Academia Sinica, Taipei 115.
J Biol Chem. 2010 Mar 26;285(13):9962-9970. doi: 10.1074/jbc.M109.087098. Epub 2010 Jan 27.
Get3, Get4, and Get5 in Saccharomyces cerevisiae participate in the insertion of tail-anchored proteins into the endoplasmic reticulum membrane. We elucidated the interaction between Get4 and Get5 and investigated their interaction with Get3 and a tetratricopeptide repeat-containing protein, Sgt2. Based on co-immunoprecipitation and crystallographic studies, Get4 and Get5 formed a tight complex, suggesting that they constitute subunits of a larger complex. In contrast, although Get3 interacted physically with the Get4-Get5 complex, low amounts of Get3 co-precipitated with Get5, implying a transient interaction between Get3 and Get4-Get5. Sgt2 also interacted with Get5, although the amount of Sgt2 that co-precipitated with Get5 varied. Moreover, GET3, GET4, and GET5 interacted genetically with molecular chaperone YDJ1, suggesting that chaperones might also be involved in the insertion of tail-anchored proteins.
在酿酒酵母中，Get3、Get4 和 Get5 参与将尾部锚定蛋白插入内质网膜的过程。我们阐明了 Get4 和 Get5 之间的相互作用，并研究了它们与 Get3 和含有四肽重复的 Sgt2 蛋白之间的相互作用。基于免疫共沉淀和晶体学研究，Get4 和 Get5 形成了紧密的复合物，表明它们构成了更大复合物的亚基。相比之下，尽管 Get3 与 Get4-Get5 复合物在物理上相互作用，但只有少量的 Get3 与 Get5 共沉淀，这表明 Get3 和 Get4-Get5 之间存在瞬时相互作用。Sgt2 也与 Get5 相互作用，尽管与 Get5 共沉淀的 Sgt2 量有所不同。此外，GET3、GET4 和 GET5 在遗传上与分子伴侣 YDJ1 相互作用，表明伴侣蛋白也可能参与尾部锚定蛋白的插入过程。
