Fernandes Jorge M O, Macqueen Daniel J, Lee Hung-Tai, Johnston Ian A
Department of Fisheries and Natural Sciences, Bodø University College, N-8049 Bodø, Norway.
Genomics. 2008 Apr;91(4):315-25. doi: 10.1016/j.ygeno.2007.10.017. Epub 2008 Jan 31.
The cee (conserved edge expressed protein) gene was recently identified in a genome-wide screen to discover genes associated with myotube formation in fast muscle of pufferfish. Comparative genomic analyses indicate that cee arose some 1.6-1.8 billion years ago and is found as a single-copy gene in most eukaryotic genomes examined. The complexity of its structure varies from an intronless gene in yeast and tunicates to nine exons and eight introns in vertebrates. cee is particularly conserved among vertebrates and is located in a syntenic region within tetrapods and between teleosts and invertebrates. Low dN/dS ratios in the cee coding region (0.02-0.09) indicate that the Cee protein is under strong purifying selection. In Atlantic salmon, cee is expressed in the superficial layers of developing organs and tissues. These data, together with functional screens in yeast and Caenorhabditis elegans, indicate that cee has a hitherto uncharacterized role in normal growth and development.
cee（保守边缘表达蛋白）基因最近在全基因组筛选中被鉴定出来，目的是发现与河豚快肌中肌管形成相关的基因。比较基因组分析表明，cee大约在16亿至18亿年前出现，并且在所检测的大多数真核生物基因组中以单拷贝基因形式存在。其结构的复杂性有所不同，从酵母和被囊动物中的无内含子基因到脊椎动物中的九个外显子和八个内含子。cee在脊椎动物中尤其保守，位于四足动物内部以及硬骨鱼和无脊椎动物之间的同线区域。cee编码区的低dN/dS比率（0.02 - 0.09）表明Cee蛋白受到强烈的纯化选择。在大西洋鲑鱼中，cee在发育中的器官和组织的表层表达。这些数据，连同在酵母和秀丽隐杆线虫中的功能筛选，表明cee在正常生长和发育中具有迄今未被表征的作用。
