Department of Urology, West China Hospital, Sichuan University, Chengdu, 610041, People's Republic of China.
In Vitro Cell Dev Biol Anim. 2010 May;46(5):408-10. doi: 10.1007/s11626-009-9264-8. Epub 2010 Jan 28.
Xenotransplantation shows great promise for providing a virtually limitless supply of cells, tissues, and organs for a variety of therapeutical procedures. However, the potential of porcine endogenous retrovirus (PERV) as a human-tropic pathogen, particularly as a public health risk, is a major concern for xenotransplantation. This study focus on the detection of copy number in various tissues and organs in Banna Minipig Inbreed (BMI) from 2006 to 2007 in West China Hospital, Sichuan University. Real-time quantitative polymerase chain reaction (SYBR Green I) was performed in this study. The results showed that the pol gene had the most copy number in tissues compared with gag, envA, and envB. Our experiment will offer a rapid and accurate method for the detection of the copy number in various tissues and was especially suitable for the selection of tissues or organs in future clinical xenotransplantation.
异种移植在提供各种治疗程序所需的几乎无限量的细胞、组织和器官方面具有巨大的潜力。然而，猪内源性逆转录病毒（PERV）作为一种人类病原体的潜在风险，特别是作为公共卫生风险，是异种移植的主要关注点。本研究专注于 2006 年至 2007 年在四川大学华西医院对来自巴纳小型猪近交系（BMI）的各种组织和器官中拷贝数的检测。本研究中进行了实时定量聚合酶链反应（SYBR Green I）。结果表明，与 gag、envA 和 envB 相比，pol 基因在组织中的拷贝数最多。我们的实验将为检测各种组织中的拷贝数提供一种快速准确的方法，特别适合于未来临床异种移植中组织或器官的选择。
