Department of Chemistry, Indian Institute of Technology, Roorkee, 247667, India.
Amino Acids. 2010 Jul;39(2):549-54. doi: 10.1007/s00726-010-0472-z. Epub 2010 Jan 28.
Indirect enantioresolution of 15 primary and secondary amino group containing compounds (amino alcohols, non-protein amino acids, PenA) was done using the reagent (S)-N-(4-Nitrophenoxycarbonyl) phenylalanine methoxyethyl ester [(S)-NIFE] by reversed-phase high-performance liquid chromatography. The diastereomeric derivatives were analyzed under reversed-phase conditions using linear gradient. The detection was at 205 nm and sharp peaks were obtained. The reagent used is comparatively economic than the other derivatizing reagents. Method validation was also done.
采用(S)-N-(4-硝基苯甲酰基)苯丙氨酸甲氧基乙酯[(S)-NIFE]通过反相高效液相色谱法对 15 种含 1 个和 2 个手性中心的化合物(氨基醇、非蛋白氨基酸、PenA)进行了间接对映体拆分。在反相条件下使用线性梯度分析非对映异构体衍生物。检测波长为 205nm,得到了尖锐的峰。所用试剂比其他衍生试剂更经济。同时也进行了方法验证。