Péter Antal, Vékes Erika, Tóth Géza, Tourwé Dirk, Borremans Frans
Department of Inorganic and Analytical Chemistry, University of Szeged, Dóm tér 7, H-6720, Szeged, Hungary.
J Chromatogr A. 2002 Mar 1;948(1-2):283-94. doi: 10.1016/s0021-9673(01)01475-3.
A new chiral derivatizing agent, (S)-N-(4-nitrophenoxycarbonyl)phenylalanine methoxyethyl ester, (S)-NIFE, was applied for the high-performance liquid chromatographic separation of enantiomers of 19 unnatural secondary amino acids: proline, pipecolic acid analogues, piperazine-2-carboxylic acid, morpholine-3-carboxylic acid, thiomorpholine-3-carboxylic acid and analogues containing the 1,2,3,4-tetrahydroisoquinoline, 1,2,3,4-tetrahydronorharmane, 1,2,3,4-tetrahydro-2-carboline and 2-benzazepine skeletons. Excellent resolutions were achieved for most of the investigated compounds by using a reversed-phase mobile phase system. The conditions of separation were optimized by variation of the mobile phase composition.
一种新型手性衍生化试剂，（S）-N-（4-硝基苯氧基羰基）苯丙氨酸甲氧基乙酯，（S）-NIFE，被用于19种非天然仲氨基酸对映体的高效液相色谱分离：脯氨酸、哌啶酸类似物、哌嗪-2-羧酸、吗啉-3-羧酸、硫代吗啉-3-羧酸以及含有1,2,3,4-四氢异喹啉、1,2,3,4-四氢去甲哈尔满、1,2,3,4-四氢-2-咔啉和2-苯并氮杂䓬骨架的类似物。通过使用反相流动相系统，大多数被研究的化合物都获得了优异的分离度。通过改变流动相组成对分离条件进行了优化。
