Department of Chemical Engineering, McGill University, 3610 University, Montreal, QC H3A 2B2, Canada.
Ann Biomed Eng. 2010 Apr;38(4):1451-62. doi: 10.1007/s10439-010-9934-4. Epub 2010 Jan 28.
Dextran is commonly used to alter growth medium rheological properties for in vitro flow experiments in order to match physiological parameters. Despite its acceptance in literature, few studies have examined dextran effects on cells. In this study, we investigated changes in endothelial cell function due to dextran, under static and flow conditions, in a concentration and time-dependent manner. Dextran increased endothelial cell viability, decreased their ability to attach to culture plates and decreased leukocyte adhesion to endothelial cells. Under static conditions, dextran increased protein and mRNA expression of intercellular adhesion molecule 1 (ICAM-1) and vascular cell adhesion molecule 1 (VCAM-1) in a concentration and time-dependent manner and caused the nuclear translocation of NF-kappaB. Steady laminar wall shear stress modulated the effects of dextran on ICAM-1, VCAM-1, and NF-kappaB expression in straight/tubular in vitro models. When the expression was normalized to their respective time matched static dextran control, it did not affect the ability to detect changes caused by shear on the mRNA expression of ICAM-1 and VCAM-1. This study demonstrates that dextran can alter endothelial cell function and therefore, caution is advised and time matched dextran controls are necessary when using dextran for dynamic cell studies.
葡聚糖常用于改变体外流动实验的生长介质流变特性，以匹配生理参数。尽管在文献中被广泛接受，但很少有研究检查葡聚糖对细胞的影响。在这项研究中，我们以浓度和时间依赖的方式研究了葡聚糖对静态和流动条件下内皮细胞功能的影响。葡聚糖增加了内皮细胞的活力，降低了它们在培养板上的附着能力，并降低了白细胞与内皮细胞的黏附。在静态条件下，葡聚糖以浓度和时间依赖的方式增加了细胞间黏附分子 1 (ICAM-1)和血管细胞黏附分子 1 (VCAM-1)的蛋白和 mRNA 表达，并导致核因子 kappaB 的核转位。稳态层流壁切应力调节葡聚糖对直/管状体外模型中 ICAM-1、VCAM-1 和 NF-kappaB 表达的影响。当将表达归一化为与其各自的静态葡聚糖对照匹配的时间时，它不会影响检测剪切对 ICAM-1 和 VCAM-1 的 mRNA 表达变化的能力。本研究表明，葡聚糖可以改变内皮细胞的功能，因此，在使用葡聚糖进行动态细胞研究时应谨慎，并需要进行时间匹配的葡聚糖对照。
