Constantinescu Ileana, Nedelcu Filofteia, Toader Maria Adela, Daniela Vasile
Molecular Biology Diagnostics Department, Centre for Immunogenetics and Virology, Fundeni Clinical Institute 258 Fundeni Av., Sector 2, Bucharest, Romania.
J Med Life. 2008 Apr-Jun;1(2):165-73.
In a country with a high prevalence (16%) of chronic serum HBsAg carriers like Romania there is a special interest in the diagnosis, epidemiology, clinics, pathology and treatment of HBV infection The idea of HBV genotyping arose from the need of understanding the complex interactions between virus and host. The purpose of this article is to present a study which aimed to identify the circulating HBV genotypes in Romania. correlate them with the clinical outcome and by HBV genotyping, to make a selection of patients for the most appropriate antiviral therapy. 130 patients were selected from different areas of the spectrum of HBV infection in which a quantitative determination of HBV-DNA was performed. HBV A genotype is associated with the inactive carrier status: a symptomatic HBV-HDV was identified in the double infection. The HBV D genotype has the most common HBV genotype (66%) and is associated with active viral infection and hepatocellular carcinoma. Long term HBV chronic infection revealed a mixture of A and D genotypes in most cases. For a proper selection of patient for the antiviral therapy, we should mandatorily genotype the HBV virus before the onset of treatment and all genotyping data must be correlated with liver biopsy assessments.
在罗马尼亚这样一个慢性血清乙肝表面抗原携带者患病率较高（16%）的国家，人们对乙肝病毒感染的诊断、流行病学、临床症状、病理学及治疗有着特殊的兴趣。乙肝病毒基因分型的想法源于理解病毒与宿主之间复杂相互作用的需求。本文的目的是呈现一项旨在识别罗马尼亚境内流行的乙肝病毒基因型的研究，将其与临床结果相关联，并通过乙肝病毒基因分型为患者选择最合适的抗病毒治疗方案。从乙肝病毒感染谱的不同区域选取了130名患者，对其进行了乙肝病毒脱氧核糖核酸的定量测定。乙肝A基因型与非活动性携带者状态相关；在双重感染中鉴定出了1例有症状的乙肝-丁肝病毒感染。乙肝D基因型是最常见的乙肝病毒基因型（66%），与活动性病毒感染和肝细胞癌相关。大多数情况下，长期乙肝慢性感染显示A和D基因型混合存在。为了正确选择抗病毒治疗的患者，我们必须在治疗开始前对乙肝病毒进行基因分型，并且所有基因分型数据都必须与肝活检评估相关联。
