Harrison T J, Anderson M G, Murray-Lyon I M, Zuckerman A J
J Hepatol. 1986;2(1):1-10. doi: 10.1016/s0168-8278(86)80002-2.
DNA-DNA hybridisation was used to examine 160 liver biopsies for the presence of the hepatitis B virus genome. HBeAg-positive HBsAg carriers were found to have replicating viral DNA in the hepatocytes and, very occasionally, HBV DNA was also integrated into the chromosomes. A high proportion of the anti-HBe-positive HBsAg carriers also have replicating HBV DNA and in the remainder integrated sequences are often, but not always, seen. No evidence was found, however, to implicate HBV in HBsAg-negative patients with alcoholic liver disease, nor in patients with a variety of other liver diseases including non-A, non-B hepatitis.
采用DNA-DNA杂交技术检测160份肝活检标本，以确定是否存在乙肝病毒基因组。发现HBeAg阳性的HBsAg携带者的肝细胞中有正在复制的病毒DNA，而且极偶尔地，乙肝病毒DNA也整合到染色体中。很大比例的抗-HBe阳性HBsAg携带者也有正在复制的乙肝病毒DNA，在其余携带者中，常常（但并非总是）可见整合序列。然而，未发现证据表明乙肝病毒与HBsAg阴性的酒精性肝病患者有关，也未发现与包括非甲非乙型肝炎在内的其他各种肝病患者有关。
