Brehar F M, Ciurea A V, Chivu Mihaela, Zarnescu Otilia, Radulescu R, Dragu Denisa
Emergency Clinical Hospital "Bagdasar-Arseni, Berceni Street 10-12, Bucharest, Romania.
J Med Life. 2008 Jul-Sep;1(3):275-86.
The inefficacity of the actual therapies for glioblastoma multiformis stimulates the researchers to search for new and innovative therapies. Therefore, the development of in vivo model for glioblastoma is an essential step during these researches, being a link between cells cultures studies and the first phases of clinical trials. In this paper, we present several procedures which have been performed for the first time in our country, such as: the cultivation and manipulation of U87MG line, the manipulation of athymic knock-out mice (NUDE Crl: CD-1 Foxn 1), the stereotactic inoculation of glioblastoma cells and finally the development of glioblastoma xenograft in the brain of inoculated nude mice. These results, which offer to the researchers from our country an in vivo model for glioblastoma, could be the start point for several projects oriented to the development of new therapies in glioblastoma, and could raise the performance of our scientific research to the European level.
多形性胶质母细胞瘤现有治疗方法的无效性促使研究人员寻找新的创新疗法。因此，胶质母细胞瘤体内模型的开发是这些研究中的关键步骤，它是细胞培养研究与临床试验第一阶段之间的纽带。在本文中，我们介绍了在我国首次进行的几种操作，例如：U87MG细胞系的培养与操作、无胸腺基因敲除小鼠（裸鼠Crl: CD-1 Foxn 1）的操作、胶质母细胞瘤细胞的立体定向接种以及最终在接种裸鼠脑内建立胶质母细胞瘤异种移植模型。这些结果为我国研究人员提供了一个胶质母细胞瘤体内模型，可能成为多个旨在开发胶质母细胞瘤新疗法项目的起点，并能将我们的科研水平提升至欧洲水平。
