Laboratory of Molecular and Cellular Biology, Faculdade de Medicina da Universidade de São Paulo - São Paulo/SP, Brazil.
Clinics (Sao Paulo). 2010 Mar;65(3):305-9. doi: 10.1590/S1807-59322010000300011.
Astrocytic gliomas are the most common intracranial central nervous system neoplasias, accounting for about 60% of all primary central nervous system tumors. Despite advances in the treatment of gliomas, no effective therapeutic approach is yet available; hence, the search for a more realistic model to generate more effective therapies is essential.
To develop an experimental malignant astrocytoma model with the characteristics of the human tumor.
Primary cells from subcutaneous xenograft tumors produced with malignant astrocytoma U87MG cells were inoculated intracerebrally by stereotaxis into immunosuppressed (athymic) Rowett rats.
All four injected animals developed non-infiltrative tumors, although other glioblastoma characteristics, such as necrosis, pseudopalisading cells and intense mitotic activity, were observed.
A malignant astrocytoma intracerebral xenograft model with poorly invasive behavior was achieved in athymic Rowett rats. Tumor invasiveness in an experimental animal model may depend on a combination of several factors, including the cell line used to induce tumor formation, the rat strains and the status of the animal's immune system.
星形胶质细胞瘤是最常见的颅内中枢神经系统肿瘤，约占所有原发性中枢神经系统肿瘤的 60%。尽管在治疗胶质瘤方面取得了进展，但目前尚无有效的治疗方法；因此，寻找一种更现实的模型来产生更有效的治疗方法是至关重要的。
建立具有人类肿瘤特征的实验性恶性星形细胞瘤模型。
通过立体定向将源自恶性星形细胞瘤 U87MG 细胞皮下异种移植肿瘤的原代细胞接种到免疫抑制（无胸腺）罗特大鼠的脑内。
所有 4 只接受注射的动物均产生了非浸润性肿瘤，尽管观察到其他胶质母细胞瘤的特征，如坏死、假栅状细胞和强烈的有丝分裂活性。
在无胸腺罗特大鼠中成功建立了具有低侵袭性行为的恶性星形细胞瘤脑内异种移植模型。实验动物模型中的肿瘤侵袭性可能取决于多种因素的组合，包括用于诱导肿瘤形成的细胞系、大鼠品系和动物免疫系统的状态。
Zotero 插件